Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-07-29
2008-07-29
Chunduru, Suryaprabha (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024310, C536S024320, C536S024330
Reexamination Certificate
active
07405040
ABSTRACT:
Described is a method for detecting 5-methylcytosine in genomic DNA samples. First, a genomic DNA from a DNA sample is chemically converted with a reagent, 5-methylcytosine and cytosine reacting differently, and the pretreated DNA is subsequently amplified using a polymerase and at least one primer. In the next step, the amplified genomic DNA is hybridized to at least two oligonucleotides, the latter being joined by inserting at least one oligonucleotide. In the ligation product, one nucleotide carries a detectable label, and the elongation depends on the methylation status of the specific cytosine in the genomic DNA sample. In the next step, the elongated oligonucleotides are analyzed for the presence of the label.
REFERENCES:
patent: 4988617 (1991-01-01), Landegren et al.
patent: 5679524 (1997-10-01), Nikiforov et al.
patent: 5728526 (1998-03-01), George, Jr. et al.
patent: 6013431 (2000-01-01), Soderlund et al.
patent: 6027889 (2000-02-01), Barany et al.
patent: 6214556 (2001-04-01), Olek et al.
patent: 7118868 (2006-10-01), Berlin
patent: 2003/0129620 (2003-07-01), Olek et al.
patent: WO 95/21271 (1995-08-01), None
patent: WO 99/28498 (1999-06-01), None
patent: WO 99/67414 (1999-12-01), None
Grunau et al., “Bisulfite genomic sequencing: systematic investigation of critical experimental parameters,” Nucleic Acids Research, 29(13):e65 (2001).
Oakeley, “DNA methylation analysis: a review of current technologies,” Pharmacology & Therapeutics, 84:389-400 (1999).
Gonzalgo et al., Nucleic Acids Research, 25(12):2529-31 (1997).
Xiong et al., Nucleic Acids Research, 25(12):2532-4 (1997).
Grigg et al., BioEssays, 16(6):431-6 (1994).
Berlin Kurt
Olek Alexander
Chunduru Suryaprabha
Epigenomics AG
Kriegsman & Kriegsman
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