Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-09-15
1999-09-14
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 436501, 935 77, 935 78, C12Q 168
Patent
active
059521740
ABSTRACT:
A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
REFERENCES:
patent: 4656127 (1987-04-01), Mundy
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5175082 (1992-12-01), Jeffreys
patent: 5185243 (1993-02-01), Ullman et al.
patent: 5427930 (1995-06-01), Birkenmeyer et al.
Jeffreys, A.J. et al., "DNA "Fingerprints " and Segregation Analysis off Multiple Markers in Human Pedigrees," Amer. J. Hum. Genet. 39:11-24 (1986).
Mullis, K.B., "The Unusual Origin of the Polymerase Chain Reaction," Scientific American Apr. 1990.
Saiki, R.K. et al., "A Novel Method for the Detection of Polymorphic Restriction Sites by Clf Oligonucleotide Probes: Application to Sickle-Cell Anemia," Bio/Technol. 3: 1008-1012 (1985).
Belle White, M. et al. "Detecting Single Base Substitutions as Heteroduplex Polymorphisms," Genomics 12:301-306 (1992).
Wu, D.Y. et al., "The Ligation Amplificait.o slashed.n Reaction (LAR)--Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation," Genomics 4:560-569 (1989).
Nickerson, D.A. et al., "Automated DNA Diagnostics Using an ELISA-based Oligonucleotide Ligation Assey," Proc. Natl. Acad. Sci. (U.S.A.) 87: 8923-8927 (1990).
Kwoh. F.Y. et al., "Transcription-Based Amplification System and Detection of Amplified Human Immunodeficiency Virus Type 1 with a Bad Sandwich Hybridization Format," Proc. Natl. Acad. Sci. (U.S.A.) 86: 1173-1177 (1989).
Frohman, M.A. et al., "Rapid Production of Full-Length CDN from Rare Transcrips: Amplification Using a Single Gene-Specific Ol Primer," Proc. Natl. Acad. Sci. (U.S.A.) 85:8998-9002 (1988).
Landegren, U. et al., "Oligonucleotide Ligation Assay," Science 241:1077-1080 (1988).
Walker, G.T. et al., "Isothermal in vitro Aplification of DNA byction enzyme/DNA polymerase System," Proc. Natl. Acad. Sci. (U.S.A.) 89:392-396 (1992).
Mathews, et al., Analytical Chem. 69:1-25(1988).
Goelet Philip
Karn Jonathan
Nikiforov Theo
Auerbach Jeffrey I.
Marschel Ardin H.
Mendelson Elliot C.
Orchid Biocomputer, Inc.
LandOfFree
Ligase/polymerase-mediated genetic bit analysis of single nucleo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Ligase/polymerase-mediated genetic bit analysis of single nucleo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ligase/polymerase-mediated genetic bit analysis of single nucleo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1509112