Ligand-mediated immunofunctional hormone binding protein assay m

Details Ligand-mediated immunofunctional hormone binding protein assay m Ligand-mediated immunofunctional hormone binding protein assay m

1990-11-19

1993-05-11

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 435 794, 435975, 436501, 436518, 436548, G01N 33543, G01N 33566, G01N 33577

Patent

active

052100170

ABSTRACT:
A ligand-mediated immunofunctional assay (LIFA) method for detecting the presence and the concentration of polypeptide hormone binding proteins which comprises capturing the binding protein with a solid phase bound first antibody, saturating the bound hormone binding protein with the ligand polypeptide hormone, and detecting the bound ligand polypeptide hormone with a detectably labeled second antibody specific for the ligand polypeptide hormone. In the absence of added saturating polypeptide hormone, the LIFA measures the amount of hormone binding protein bound to the endogenous ligand polypeptide hormone. A growth hormone binding protein assay illustrates the method of the present invention.

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