Ligand analog-irreversible enzyme inhibitor conjugates

Details Ligand analog-irreversible enzyme inhibitor conjugates Ligand analog-irreversible enzyme inhibitor conjugates

1981-01-26

1985-10-29

Sutto, Anton H.

Organic compounds -- part of the class 532-570 series

Organic compounds

Four or more ring nitrogens in the bicyclo ring system

260944, 2603974, 2603975, 2603972, 536 136, 536 25, 548413, C07F 960, C07F 940

Patent

active

045501637

ABSTRACT:
The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction.
The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

Affiliated with

Also associated with

Rating

Ligand analog-irreversible enzyme inhibitor conjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ligand analog-irreversible enzyme inhibitor conjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ligand analog-irreversible enzyme inhibitor conjugates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-729162