Leukocyte migration through antigen containing agaroses for immu

Chemistry: analytical and immunological testing – Involving diffusion or migration of antigen or antibody

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436 63, 436 64, 436515, 436519, 436529, 436808, 436811, 436813, G01N 3348, G01N 3350, G01N 3354

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active

044368240

ABSTRACT:
The method assesses the level of general and specific cellular immunocompetence by measuring the responses of individuals to antigens in vitro employing the phenomenon of Leukocyte Migration Inhibition (LMI). The present invention differs from the previously described LMI technique in that antigens are individually incorporated into the agarose of assay plates, requiring no preincubation of antigens with patient blood cell (leukocyte) suspensions. The LMI assay method described herein is a practical alternative to delayed hypersensitivity skin testing to identify cellular immune deficiency and avoids the risk and inconvenience of the skin test procedure. The method also allows in vitro diagnosis of Tuberculosis and monitoring of tumor therapy.

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