Leukocyte differentiation method

Details Leukocyte differentiation method Leukocyte differentiation method

1987-01-28

1996-05-21

Housel, James C.

Chemistry: analytical and immunological testing

Determination of water

In petroleum oil, hydrocarbon oil or organic fluid

436 8, 436 11, 436 17, 436 18, G01N 3100

Patent

active

055189288

ABSTRACT:
A method is disclosed for differentiating white blood cells. The method uses at least one surfactant and at least one dilute acid which together selectively strip the cytoplasm from certain classes of white blood cells and not others. More particularly, the method causes lysis of lymphocytes, monocytes, eosinophils and neutrophils, but not basophils. Thus, basophils are differentiated from other PMN sub-classes by their appearance as intact cells. The method totally avoids the need for dye preparation and the vagaries of staining techniques and can be used either manually or with instrumentation.

REFERENCES:
patent: 4506018 (1985-03-01), North, Jr.
patent: 4528274 (1985-07-01), Carter et al.
patent: 4654312 (1987-03-01), Chang et al.
patent: 4751179 (1988-06-01), Ledis et al.

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