Leucocyte adherence inhibition assay for detection of cancer

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 4, 436 63, 436 64, 436519, 436800, 436813, G01N 3352, G01N 3354, G01N 3388

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044264461

ABSTRACT:
Methods for carrying the leucocyte adherence inhibition (LAI) assay for detecting the presence of cancer in responsive patients. One aspect of the invention utilizes incubation with agents which maximize the intracellular cycle 3',5'-adenosine monophosphate (cAMP) levels in the leucocytes as a means to expand the percentage of responding subjects in different cancers. A second aspect utilizes measurement of extracted, vital cell dye to avoid the need of counting the adhering or non-adhering leucocytes.

REFERENCES:
patent: 3999944 (1976-12-01), Grosser et al.
patent: 4060457 (1977-11-01), Iizuka et al.
patent: 4094745 (1978-06-01), Scholefield et al.
patent: 4254219 (1981-03-01), Fullerton
Rose et al., Methods in Immunodiagnosis, 2nd ed., John Wiley & Sons, N.Y. (1980), pp. 39-43.
Kalafut et al., "Application of the Indirect Tube LAI Assay in the Study of Cell-Medicated Immunity in Rats Immunized with B77 Tumor Cells", Chem. Absts., vol. 94, No. 19, (1981), p. 469, Absts. #154656w.
Howell, et al., "Characterization of Murine Tumor-Associated Antigens by the Microleukocyte Adherence Inhibition Assay", Chem. Absts., vol. 90, No. 13 (1979), p. 418 Absts. #101755p.
Thomson, et al., "Evidence for the Expression of Human Tumor Specific Antigens Associated with B.sub.2 -Microglobulin in Human Cancer and in some Colon Indenomes and Benign Breast Lesions", Chem. Absts., vol. 90, No. 15, (1979), pp. 482, 483 Absts. #119610x.
Powell et al., "Antigenic Specificity and Cellular Mechanisms in Leukocyte Adherence Inhibition Analysis of Immunity to Simple Proteins and Hapten-Protein Conjugates", Chem. Absts., vol. 90, No. 15, (1979), p. 467 Absts. #119477.
Bryant et al., "The Effect of 3',5'-Adenosine Monophosphate Granulocyte Adhesion", J. Chem. Invest., vol. 54 (1974), pp. 1241-1244.
Powell et al., "Leukocyte-Adherence Inhibition: A Specific Assay of Cell Medicated Immunity Dependent on Lymphokine Medicated Collaboration between T Lymphocytes", J. Immunology, vol. 120, No. 6, (1978), pp. 1957-1966.

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