Leucine dehydrogenase and a process for production thereof

Details Leucine dehydrogenase and a process for production thereof Leucine dehydrogenase and a process for production thereof

1983-08-24

1985-10-08

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435191, 435832, C12Q 132, C12N 906, C12R 107

Patent

active

045460779

ABSTRACT:
Leucine dehydrogenase is disclosed which retains 80% or more of the activity even after it is incubated in a buffer solution at about 70.degree. C. for 10 minutes, as compared to the activity prior to incubation. A process for producing a heat resistant leucine dehydrogenase is also disclosed. The process comprises culturing a microorganism having an optimum growth temperature of 50.degree. to 85.degree. C. and harvesting the leucine dehydrogenase from the culture and a composition of assaying for leucine aminopeptidase (LAP) comprising the heat resistant leucine dehydrogenase.
The leucine dehydrogenase can be stored for a long period of time and thus very effective for investigations in biochemistry, food analysis and clinical test. Further the composition provides extremely easy assay for the LAP activity in high accuracy with good reproducibility which is an important item in clinical test.

REFERENCES:
patent: 4179335 (1979-12-01), Long et al.
patent: 4331762 (1982-05-01), Nakajima et al.
patent: 4342827 (1982-08-01), Atkinson et al.
Zink et al., "L-Leucine Dehydrogenase (Bacillus Cereus) in Methods in Enzymology 17A, 799-803, (1970).

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