Lettuce plants having broad-spectrum Dm-resistance genes

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S269000, C800S298000

Reexamination Certificate

active

06903249

ABSTRACT:
A method for obtaining a plant, in particular a cultivated lettuce plant (L. sativa), with a lasting resistance to a pathogen, in particularBremia lactucae, comprising of providing one or more specific DNA markers linked to one or more resistance genes, determining the presence of one or more resistance genes in a plant using these DNA markers, subsequently crossing a first plant comprising one or more resistance genes with a second plant comprising one or more resistance genes, and selecting from the progeny a plant in which one or more resistance genes are present by using the DNA markers. The invention further relates to the plants obtained with this method, seeds and progeny of these plants, as well as progeny thereof.

REFERENCES:
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Farrara et al 1987, Plant Pathology 36: 499-514.
Maisonneuve et al 1994, Theor. Appl. Genet. 89: 96-104.
Kessell, Rick et al., “Recessive Resistance toPlasmopara lactucae-radicisMaps by Bulked Segregant Analysis to a Cluster of Dominant Disease Resistance Genes in Lettuce,”Molecular Plant-Microbe Interactions,vol. 6, No. 6, pp. 722-728, 1993.
Michelmore, R.W. et al., “Molecular Markers and Genome Analysis in the Manipulation of Lettuce Downy Mildew,”Advances in Molecular Genetics of Plant-Microbe Interactions,pp. 517-523, 1993.
Paran, I. et al., “Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce,”Theory of Applied Genetics,vol. 85, pp. 985-993, 1993.
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Landry, B. et al. “A Genetic Map of Lettuce (Lactuca sativa L.) With Restriction Fragment Length Polymorphism, Isozyme, Disease Resistance and Morphological Markers”Genetics116: 331-337 (1987).
Maisonneuve, B. “Utilisation de la culture in vitro d'embryons immatures pour les croisements interspécifiques entreLactuca sativa L.etL. saligna L.ouL. virosa L.;étude des hybrides obtenus (“Interspecific hybridization inLactucasp. using in vitro culture of immature embryos, and study of hybrid offspring”)”Agronomie7(5): 313-319 (1987) (The summary is in English).
Maisonneuve, B. et al. “Rapid mapping of two genes for resistance to downy mildew fromLactuca serriolato existing clusters of resistance genes”Theor Appl Genet89: 96-104 (1994).
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