Leptin assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 72, 435 721, 435 78, G01N 3353, G01N 3350

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active

060079983

ABSTRACT:
A trans-activation assay for leptin is described. Leptin response elements are located proximal to a promoter, and the promoter region is operatively linked to a reporter gene. When leptin binds with the receptor, the reporter gene is transcribed.

REFERENCES:
patent: 5401629 (1995-03-01), Harpold et al.
patent: 5436128 (1995-07-01), Harpold et al.
patent: 5643748 (1997-07-01), Snodgrass et al.
patent: 5698389 (1997-12-01), de la Brousse et al.
He et al, JBC 270(48) p. 2888 7-91, 1995.
Gong et al, JBC 271(8) 1996, pp. 3971-3974.
De La Brousse et al, PNAS 93, 1996, pp. 4096-4401.
Darnell et al, Science 264, 1994, pp. 1415-1420.
Shki et al, Nature 377; 1995, pp. 591-594.
Rosenblum et al. "Functional STAT 1 and 3 signaling by the leptin receptor (OB-R); reduced expression of the rat fatty leptin receptor in transfected cells," 1996 Endocrinology 137(11):5178-5181.
Andersson et al. "Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile biosynthetic enzyme," 1989 J. Biol. Chem. 264:8222-8229.
Barnes, W. M. "PCR amplification of up to 35-Kb DNA with high-fidelity and high-yield from lambda-bacteriophage," 1994 Proc. Natl. Acad. Sci. 91:2216-2220.
Baumann et al. "The full-length leptin receptor has signalling capabilities of interleukin 6-type cytokine receptors" 1996 Proc. Natl. Acad. Sci. USA 93:8374-8378.
Chehab et al. "Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin", 1996 Nature Genetics 12:318-320.
Chen et al. "Evidence that the diabetes gene encodes the leptin receptor: Identification of a mutation in the leptin receptor gene in db/db mice" 1996 Cell 84:491-495.
Cioffi et al. "Novel B219/OB receptor isoforms:Possible role of leptin in hematopoiesis and reproduction", 1996 Nature Medicine 2:585-588.
Ghilardi et al. "Defective STAT signaling by the leptin receptor in diabetic mice" 1996 Proc. Natl. Acad. Sci. USA 93:6231-6235.
Halaas et al. "Weight-reducing effects of the plasma-protein encoded by the obese gene", 1995 Science 269:543-546.
Hodgson, J. "Receptor screening and the search for new pharmaceuticals" 1992 Bio/Technology 10:973-977.
Iida et al. "Phenotype-linked amino acid alteration in leptin receptor cDNA from zucker fatty (fa/fa) rat" 1996 Biochem. Biophys. Res. Comm. 222:19-26.
Lee et al. "Abnormal splicing of the leptin receptor in diabetic mice", 1996 Nature 379:332-335.
McKnight et al. "Transcriptional control signals of a eukaryotic protein-coding gene", 1982 Science 217:316-324.
Mellon et al. "Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis", 1990 Neuron 5:1-10.
Phillips et al. "Leptin receptor missense mutation in the fatty zucker rat", 1996 Nature Genetics 13:18-19.
Pine et al. "Tyrosine-phosphorylated p91 binds to a single-element in the isgf2/irf-1 promoter to mediate induction by ifn-alpha and ifn-gamma, and is likely to autoregulate the p91 gene", 1994 Embo Journal 13:158-167.
Schadlow et al. "Regulation of gene expression in PC12 cells via an activator of dual second messengers: pituitary adenylate cyclase activating polypeptide" 1992 Mol. Biol. 3:941-951.
Sims et al. "A novel interferon-inducible domain--structural and functional--analysis of the human interferon regulatory factor-I gene promoter", 1993 Molecular And Cellular Biology 13:690-702.
Tartaglia et al. "Identification and expression cloning of a leptin receptor, ob-r", 1995 Cell 83:1263-1271.
Wang et al., "A novel leptin receptor isoform in rat", 1996 FEBS Letters 392:87-90.
Zhang et al. "Positional cloning of the mouse obese gene and its human homolog", 1994 Nature 372:425-432.
Zhong et al. "Stat-3 a stat family member activated by tyrosine phosphorylation in response to epidermal growth-factor and interleukin-6", 1994 Science 264:95-98.
Zucker and Zucker "Fat accretion and growth in the rat", 1963 J. Nutrition 80:6-19.
Zucker and Zucker "Heredity obesity in the rat associated with high serum fat and cholesterol", 1962 Proc. Soc. Exp. Miol. Med. 110:164-171.
Zucker and Zucker "Fatty, a new mutation in the art", 1961 J. Heredity 52:275-278.

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