Lentivirus vectors for gene transfer to alveolar epithelial...

Details Lentivirus vectors for gene transfer to alveolar epithelial... Lentivirus vectors for gene transfer to alveolar epithelial...

2007-03-09

2009-10-13

Chen, Shin-Lin (Department: 1632)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C424S093200, C424S093600, C435S320100, C435S455000

Reexamination Certificate

active

07601345

ABSTRACT:
The present invention demonstrates that VSV-G-pseudotyped lentivirus vectors efficiently transduce AEC in primary culture and in vivo with transduction favored by virus application from the apical side. Transduction efficiency in AEC increased with increasing MOI and greatly exceeded that achieved with a similarly pseudotyped MLV retrovirus vector. The present invention also demonstrates the successful in vivo transfer of genes through lentivirus vector transduction. Mammals injected with lentivirus vector via the trachea expressed the reporter protein in alveolar epithelial cells within 48 to 72 hours after infection.

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