Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Reexamination Certificate
2007-12-25
2007-12-25
Campell, Bruce R. (Department: 1648)
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
C424S093100, C424S069000, C435S069200, C435S320100, C435S455000, C435S456000, C435S465000, C435S466000
Reexamination Certificate
active
11099872
ABSTRACT:
Novel packaging cell lines which produce recombinant retrovirus, free of detectable helper-virus are disclosed. Also disclosed are methods of making the cell lines and methods of producing recombinant retroviruses from the cell lines. Retroviruses produced by the cell lines include lentiviruses, such as HIV, capable of transfering heterologous DNA to a wide range of non-dividing cells. The packaging cells contain at least three vectors which collectively encode retroviral gag, pol, and env proteins, wherein the gag and pol genes are separated, in part, onto two or more different vectors. This is made possible by fusing Vpr or Vpx to pol proteins separated from gag so that the proteins are targeted to assembling virions. Among other advantages, the packaging cells provide the benefit of increased safety when used in human gene therapy by virtually eliminating the possibility of molecular recombination leading to production of replication competent helper virus.
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Richardson, Jennifer H. et al, “Helper virus-free transfer of human immunodeficiency virus type 1 vectors,”Journal of General Virology, vol. 76:691-696 (1995).
Leboulch Philippe
Westerman Karen
Campell Bruce R.
DeConti, Jr. Giulio A.
Genetix Pharmaceuticals, Inc.
Horning Michelle
Lahive & Cockfield LLP
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