Leguminous bacterium having potentiated nitrogen fixation...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S410000, C435S419000, C435S420000, C435S243000, C435S252100, C435S252300

Reexamination Certificate

active

07109020

ABSTRACT:
The invention of this application provides root nodule bacteria transformed with the DNA fragment of SEQ ID No. 1 consisting a catalase gene and whose nitrogen-fixation ability is enhanced with a catalase produced from the catalase gene, a preparation for leguminous crops containing the root nodule bacteria as an active ingredient, and a method of cultivating leguminous crops which comprises contacting seeds of leguminous crops with these. These inventions enable the acceleration of growth of leguminous crops and multiplication of crop yield of beans. Further, the reduction of agricultural cost and the alleviation of environmental pollution are realized by the reduction of nitrogen fertilizers.

REFERENCES:
patent: 2000-316584 (2000-11-01), None
Yumoto et al. Journal of Fermentation and Bioengineering, vol. 85 No. 1 pp. 113-116, 1998).
Berendsen (Science. 1998, vol. 282, pp. 642-643).
Yumoto et al. (Applied and Environmental Microbiology vol. 65 No. 1, 1999, pp. 67-72).
K. Swaraj et al., “Effect of salt stress on nodulation and nitrogen fixation in legumes”, Indian J. Exp. Biol., vol. 37, No. 9, 1999, pp. 843-848.
N.R. Bishnoi et al., “Influence of sodium chloride on nitrogen fixation and enzymes assocated with scavenging hydrogen peroxide in clusterbean root modules”, Indian J. Exp. Biol., vol. 35, No. 2, 1997, pp. 193-196.
T. Ohwada et al., “Susceptibility to hydrogen peroxide and catalase activity of root nodule bacteria”, Biosci. Biotechnol. Biochem., vol. 63, No. 3, 1999.
T. Owada et al., “Rhizobium fredii USDA191 no Kasanka Suiso Kanjusei to Katalase, Peroxidase Kassei ni tsuite”, Taidai Kenpo, vol. 21, No. 1, 1998, pp. 17-25.
T. Owada et al., “Rhizobium fredii USDA191 no Kasanka Suiso Taisei Hen'ikabu no Konryu Keisei oyobi Chisso Kotei kino ni tsuite”, Taidai Kenpo, vol. 21, No. 1, 1998, pp. 27-35.

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