Leech protein having anticoagulant and antimetastatic properties

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530413, 530416, 530855, C07K 1300, C07K 302, C07K 328

Patent

active

051945894

ABSTRACT:
The present invention comprises a biochemically pure protein, termed antistasin, which exhibits both antimetastatic and anticoagulant properties. Methods of producing the protein and methods of using the protein to inhibit or reduce metastasis and/or coagulation of blood are disclosed. The complete amino acid sequences of antistasin and some variants have been determined from purified protein and cloned cDNAs.

REFERENCES:
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patent: 4588587 (1986-05-01), Gasic
Iwakawa et al. (1986) Clin. Expl. Metastasis 4(3):205-220.
Gasic et al. (1984) Problems and Proceedings, London, 275-278.
Gasic et al. (1984) Cancer Res. 44:5670-5676.
Murer et al. (1984) Thrombosis & Haemostasis 51(1):24-26.
Gasic et al. (1984) Proceedings of AACR 25: p. 58, abstract 229.
Bajkowski et al. (1984) Proceedings of AACR 25: p. 58, abstract 230.
Haementeria Officialis, International Conference, Jul. 1986, abstract.
Gasic et al. (1983) Cancer Res. 43:1633-1636.
G. J. Gasic et al., "Treatment of Experimental Metastasis by a Purified Protein from Leech Salivary Glands," in Treatment of Metastasis, Abstracts, 1st Meeting of Metastasis Research Society, Apr. 3-4, 1986, p. 25.
G. J. Gasic, et al., "Isolation of Protein from Salivary Glands of the Leech Haementeria Officinalis with Potent Antimetastatic and Anticoagulant Activity", in Proceedings of AACR, vol. 27, No. 261, Mar. 1986.
G. J. Gasic, "Leeches in Cancer Research: Isolation of a Purified Protein From Salivary Gland Extracts (SGE) of Haementeria Officinalis with Potent Antimetastatic and Anticoagulant Activities", British Association of Leech Scientists, International Conference, Jul. 11-12, 1986.
Scott et al, "Protease Nexin Properties and a Purification Procedure," Journal of Biological Chemistry, vol. 260, No. 11, pp. 7029-7034, Jun. 10, 1989.
Scott et al., "Purification of Human Protease Nexin," in The Journal of Biological Chemistry, vol. 258, No. 17, pp. 10439-10444, Sep. 10, 1983.
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