Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-03-04
1993-09-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 794, 436518, 436813, G01N 33577, G01N 33574, G01N 33566, G01N 33536
Patent
active
052427994
ABSTRACT:
A heterologous sandwich immunoassay has been developed for human TF antigen using a monoclonal antibody as the catcher and labelled peanut agglutinin as the probe. Since human TF antigen is shed by tumor cells, a positive determination of the TF antigen in a patient sample indicates the presence of cancer. The test can also be used to monitor the course of conventional chemotherapy or radiotherapy by monitoring the amount of TF antigen in a fluid sample from a patient being so treated. Because of the sensitivity of the assay method, cancer can be detected in very early stages by the assay method of the present invention.
REFERENCES:
patent: 4818682 (1989-04-01), Linnane
patent: 5051354 (1991-09-01), Henslee et al.
Springer, et al., Science, 224:1198-1206 (1984); T and Tn, General Carcinoma Autoantigens.
Samuel, et al., Cancer Research, 50:4801-08 (1990); Analysis of Human Tumor Associated Thomsen-Friedenreich Antigen.
Itzkowitz, et al., Cancer Research, 49:197-204; (1989) Expression of Tn, Sialosyl-Tn, and T Antigens in Human Colon Cancer.
N. Shimoda et al., J. Biochem. 102: 657-664, 1987.
Gold and Miller, Nature 255: 85-87 1 May 1975.
Boland et al., Proc. Natl. Acad. Sci. USA 79: 2051-2055, Mar. 1982.
Wolf et al. Specificity of Reagents Directed to the Thomsen-Friedenreich Antigen and Their Capacity to Bind to the Surface of Human Carcinoma Cell Lines, Cancer Research 46:1779-1782 (1986).
Yuan, et al. Comparison of T-Antigen Expression in Normal, Premalignant, and Malignant Human Colonic Tissue Using Lectin and Antibody Immunohistochemistry, Cancer Research 46:4841-4847 (1986).
Longenecker B. Michael
Samuel John
Biomira, Inc.
Cooper Iver P.
Walsh Stephen
Wax Robert A.
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