Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1988-04-14
1991-01-01
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530395, 530413, 530417, 530415, C07K 318, C07K 320
Patent
active
049819518
ABSTRACT:
A method of purifying a recombinant protein from a solution, such as tissue culture fluid, containing gylcoproteins. The affinity of lectins for specific glycoproteins is assessed and used to select a particular lectin specific for the contaminating glycoprotein(s). A sugar buffer such as alpha methyl mannoside prevents binding of the recombinant protein. The preferred lectin is lentil lectin, for use in separating recombinant Factor VIII from tissue culture fluid contaminated with rodent protein from the cell line used to produce the recombinant Factor VIII.
REFERENCES:
patent: 4341754 (1982-07-01), Kaplan et al.
patent: 4886747 (1989-12-01), Derynck et al.
Aston David J.
Bradley Bertram
Furman Keith C.
Miles Inc.
Moskowitz Margaret
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