Leafy cotyledon2 genes and their uses

Details Leafy cotyledon2 genes and their uses Leafy cotyledon2 genes and their uses

2000-03-16

2002-12-10

McElwain, Elizabeth F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters plant part growth

C800S287000, C800S278000, C800S298000, C536S023100, C536S024100, C536S023600, C435S419000, C435S468000

Reexamination Certificate

active

06492577

ABSTRACT:

FIELD OF THE INVENTION
The present invention is directed to plant genetic engineering. In particular, it relates to new embryo-specific genes useful in improving agronomically important plants.
BACKGROUND OF THE INVENTION
Embryogenesis in higher plants is a critical stage of the plant life cycle in which the primary organs are established. Embryo development can be separated into two main phases: the early phase in which the primary body organization of the embryo is laid down and the late phase which involves maturation, desiccation and dormancy. In the early phase, the symmetry of the embryo changes from radial to bilateral, giving rise to a hypocotyl with a shoot meristem surrounded by the two cotyledonary primordia at the apical pole and a root meristem at the basal pole. In the late phase, during maturation the embryo achieves its maximum size and the seed accumulates storage proteins and lipids. Maturation is ended by the desiccation stage in which the seed water content decreases rapidly and the embryo passes into metabolic quiescent state. Dormancy ends with seed germination, and development continues from the shoot and the root meristem regions.
The precise regulatory mechanisms that control cell and organ differentiation during the initial phase of embryogenesis are largely unknown. The plant hormone abscisic acid (ABA) is thought to play a role during late embryogenesis, mainly in the maturation stage by inhibiting germination during embryogenesis (Black, M. (1991). In Abscisic Acid: Physiology and Biochemistry, W. J. Davies and H. G. Jones, eds. (Oxford: Bios Scientific Publishers Ltd.), pp. 99-124); and Koornneef, M., and Karssen, C. M. (1994). In Arabidopsis, E. M. Meyerowitz and C. R. Sommerville, eds. (Cold Spring Harbor: Cold Spring Harbor Laboratory Press), pp. 313-334). Mutations that effect seed development and are ABA insensitive have been identified in Arabidopsis and maize. The ABA insensitive (abi3) mutant of Arabidopsis and the viviparous1 (vp1) mutant of maize are detected mainly during late embryogenesis (McCarty, et al. (1989)
Plant Cell
1:523-532 and Parcy et al. (1994)
Plant Cell
6:1567-1582). Both the VP1 gene and the ABI3 genes have been isolated and were found to share conserved regions (Giraudat, J. (1995)
Current Opinion in Cell Biology
7:232-238 and McCarty, D. R. (1995).
Annu. Rev. Plant Physiol. Plant Mol. Biol.
46:71-93). The VP1 gene product has been shown to function as a transcription activator (McCarty, et al. (1991)
Cell
66:895-906). It has been suggested that ABI3 has a similar function.
In spite of the recent progress in defining the genetic control of embryo development, further progress is required in the identification and analysis of genes expressed specifically in the embryo and seed. Characterization of such genes would allow for the genetic engineering plants with a variety of desirable traits. For instance, modulation of the expression of genes that control embryo development may be used to alter traits such as accumulation of storage proteins in leaves and cotyledons. Alternatively, promoters from embryo or seed-specific genes can be used to direct expression of desirable heterologous genes to the embryo or seed. The present invention addresses these and other needs.


REFERENCES:
patent: WO 98/37184 (1998-08-01), None
Broun et.al.; Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids, 1998, Science, vol. 282: 1315-1317.*
Hill et. al.; Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia Coli, 1998, Biochemical and Biophsysical Research Communications 244: 573-577.*
Izawa et. al.; Plant bZIP Protein DNA Binding Specificity, 1993, J. Moi. Biol. 230: 1131-1144.
Bowie et.al.; Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions, 1990, Science, vol. 247:1360-1310.
Burgess et.al.; Possible Dissociation of the Heparin-binding and MitogenicActivities . . . by Site-directed Mutagenesis of a Single Lysine Residue, 1990, Journal of Cell Biology, vol. 111: 2129-2138.
Lazar et.al.; Transforming Growth Factor: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities, 1988, Molecular and Cellular Biology: 1247-1252.
Lohmann et.al.; A Molecular Link between Stem Cell Regulation and Floral Patterning in Arabidopsis, 2001, Cell, vol. 105: 793-803.
Busch et.al.; Activation of a Floral Homeotic Gene in Arabidopsis, 1999, Science, vol. 285: 585-587.
Stone et.al.; Leafy Cotyledon2 encodes a B3 domain transcription factor that induces embryo development, 2001,PNAS, vol. 98, No. 20:11806-11811.
Hao et.al.; Unique Mode of GCC Box Recognition by the DNA-binding Domain of Ethylene-responsive Element-binding Factor (ERF Domain) in Plant, 1998, Journal of Biological Chemistry, vol. 273,No. 41: 26857-26861.
West, et al., “Leafy Coyledon1 Is An Essential Regulator of Late Embryogenesis and Cotyledon Identity in Arabidopsis”;The Plant Cell, vol. 6, 1731-1745 (Dec. 1994).
Meinke, et al., “Leafy Cotyledon Mutants of Arabidopsis”;The Plant Cell, vol. 6, 1049-1064 (Aug. 1994).
Baumlein, et al., “The FUS3 Gene ofArabidopsis thalianais a regulator of gene expression during late embryogenesis,”;The Plant Journal, 6(3): 379-387 (1994).
Becker, et al., “A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast”;Proc. Natl. Acad. Sci. USA, vol. 88:1968-1972 (Mar. 1991).
Sinha, et al., “Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3”;Proc. Natl. Acad. Sci. USA, vol. 92, 1624-1628 (Feb. 1995).
Li, et al., “Evolutionary variation of the CCAAT-binding transcription factor NF-Y”;Nucleic Acids Res., vol. 20, No. 5, 1087-1091 (Feb. 1992).
Johnson, et al., “Eukaryotic Transcriptional Regulatory Proteins”;Annu. Rev. Biochem., 58:799-839 (1989).
Xing, et al. “Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain”;The EMBO J., vol. 12, No. 12, 4647-4655 (1993).
McCarty, et al., “The Viviparous-1 Developmental Gene of Maize Encodes a Novel Transcriptional Activator”;Cell, vol. 66, 895-905 (Sep. 1991).
McCarty, D., “Genetic Control and Integration of Maturation and Germination Pathways in Seed Development”;Annu. Rev. Plant Physiol. Plant Mol. Biol.,46:71-93 (1995).
Giraudat, J., “Abscisic acid signaling”;Current Opinion in Cell Biology, 7:232-238 (1995).
Parcy, et al., “Regulation of Gene Expression Programs during Arabidopsis Seed Development: Role of the AB13 Locus and Endogenous Abscisic Acid”;The Plant Cell, vol. 6, 1567-1582 (Nov. 1994).
McCarty, et al., “Molecular Analysis of viviparous-1: An Abscisic Acid-Insensitive Mutant of Maize”;The Plant Cell, vol. 1, 523-532 (May 1989).
Heck, et al., “AGL15, a MADS Domain Protein Expressed in Developing Embryos”;The Plant Cell, vol. 7, 1271-1282 (Aug. 1995).
Valvekens, et al., “Agrobacterium tumefaciens-mediated transformation ofArabidopsis thalianaroot explants by using kanamycin selection”;Proc. Natl. Acad. Sci., USA, vol. 85, 5536-5540 (Aug. 1988).
Ming-Tsair Chan, et al., “Novel Gene Expression System for Plant Cells Based on Induction of &agr;-Amylase Promoter by Carbohydrate Starvation”;The Journal of Biological Chemistry, vol. 269, No. 26, 17635-17641 (Jul. 1994).
Shimada, et al., “Antisense regulation of the rice waxy gene expression using a PCR-amplified fragment of the rice genome reduces the amylose content in grain starch”;Theoretical and Applied Genetics, vol. 86, 665-672 (Jan. 1993).
Meinke, David, “A Homoeotic Mutant ofArabidopsis thalianawith Leafy Cotyledons”;Science, vol. 258, 1647-1650, (Dec. 1992).
Keith, K., et al. “fusca3: A Heterochronic Mutation Affecting Late Embryo Development in Arabidopsis,”Plant Cell6:589-600 (May 1994).
Lotan, T., et al. “Arabidopsis Leafy Cotyledon1 Is Sufficient to Induce Embryo Development in Vegetative Cells,”Cell93: 1195-1205 (Jun. 1998).
LuerBen, H., et al. “Fusca3 encodes a protein with a conserved VP1/ABI3-like B3 domain which is of functional importance for the regulati

Affiliated with

Also associated with

Rating

Leafy cotyledon2 genes and their uses does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Leafy cotyledon2 genes and their uses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Leafy cotyledon2 genes and their uses will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2960928