Layered sandwich assay method for chlamydia and materials theref

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 791, 435 794, 435 18, 435 19, 435 21, 435 24, 435975, 436518, 436532, G01N 33571, G01N 33543

Patent

active

051889374

ABSTRACT:
An assay for Chlamydia includes contacting Chlamydia organisms in a liquid with a solid support having an antispecies Fe antibody immobilized thereon and an anti-Chlamydia capture antibody. After binding of Chlamydia antigen to the capture antibody and binding of the capture antibody to the antispecies antibody on the support, a tracer including a label conjugated to a signal antibody is added. After binding of the signal antibody to the antigen, the presence of Chlamydia organisms in the liquid is detected by a signal associated with the label thereby bound to the support. The invention includes a kit of materials for performing an assay according to the method of the invention.

REFERENCES:
patent: 4092408 (1978-05-01), Litt
patent: 4497899 (1985-02-01), Armstrong et al.
patent: 4525452 (1985-06-01), Jones et al.
patent: 4663291 (1987-05-01), Rose
patent: 4683196 (1987-07-01), McLaughlin
patent: 4835099 (1989-05-01), Mize et al.
Peterman et al, J. Immunol. Methods, 111:271-275 (1988).
McLean, American Biotechnology Laboratory, 10(5):58 (May 1992).
Conradie et al, J. Immunol. Methods. 59:289-299 (1983).
Lin et al. J. Immunol. Methods, 125:67-77 (1989).
Suter et al, Immunology Letters, 13:313-316 (1986).
Butler, Ed., Immunochemistry of Solid-Phase Immunoassay, CRC Press, pp. 3-14, 221-231 (1991).
Sankoili et al, "Improvement in the Antibody Binding Characteristics of Microtitre Wells by Pretreatment with Anti-IgG Fc Immunoglobulin", J. Immunol. Methods 104:191-194 (1987).
Schoenwald, E., et al, Biological Abstracts, vol. 86(2), 1988, Abstract No. 16541.
Barnes, R. C. et al, Biological Abstracts, vol. 81(3), 1986, Abstract no. 24391.
Ash, C., Chemical Abstracts, vol. 108, 1988, Abstract No. 184747b.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Layered sandwich assay method for chlamydia and materials theref does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Layered sandwich assay method for chlamydia and materials theref, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Layered sandwich assay method for chlamydia and materials theref will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2204110

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.