Layered immunoassay using antibodies bound to transport particle

Details Layered immunoassay using antibodies bound to transport particle Layered immunoassay using antibodies bound to transport particle

1986-06-09

1989-06-06

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 55, 422 56, 422 58, 422 60, 435287, 435288, 435310, 436523, 436541, 436807, 436819, 436824, 436828, G01N 3353

Patent

active

048371459

ABSTRACT:
A device is provided for determining the presence of an antigen which comprises a trapping zone, which contains material capable of capturing free flowing enzyme linked antibodies, but not antibodies bound to a transport particle which flows freely through the trapping zone into the substrate zone, and a substrate zone which contains material capable of reacting with enzyme-linked antibodies to produce a reaction which indicates the presence of antibodies. A method of determining the presence of an antigen is provided wherein a sample is mixed with two classes of antibodies which are specific for the antigen being tested for, but which react with different antigen domains, wherein the mixture consists of class one antibodies bound to a transport particle which flows freely through the trapping zone and class two enzyme-linked antibodies which are incapable, unless bound to the transport particles, of flowing freely through the trapping zone. In the presence of the antigen being tested for, both classes of antibodies bind to the antigen and flow through the trapping zone into the substrate zone, wherein a reaction takes place to indicate the presence of the antigen.

REFERENCES:
patent: 4459358 (1984-07-01), Berke
patent: 4459361 (1984-07-01), Gefter
patent: 4666863 (1987-05-01), Edwards et al.

Affiliated with

Also associated with

Rating

Layered immunoassay using antibodies bound to transport particle does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Layered immunoassay using antibodies bound to transport particle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Layered immunoassay using antibodies bound to transport particle will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-40244