Lawn assay for compounds that affect enzyme activity or bind to

Details Lawn assay for compounds that affect enzyme activity or bind to Lawn assay for compounds that affect enzyme activity or bind to

1995-11-03

1999-01-05

Duffy, Patricia

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 71, 435 72, 435 78, 435177, 435178, 435182, 436164, 436169, 436170, C12Q 100

Patent

active

058560831

ABSTRACT:
A lawn assay is described for determining compounds that affect enzyme activity or that bind to target molecules. Compounds to be screened are cleaved, and diffused from solid supports into a colloidal matrix. Enzymatic catalysis or binding to target molecules by the compounds is carried out in the matrix. Active compounds are found by monitoring a photometrically detectable change in a substrate, coenzyme, or cofactor involved in the enzymatic reaction, or in a labeled ligand bound to the target molecule, that produces a zone of activity associated with the compounds.

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