Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – Enzyme component of specific activity or source
Reexamination Certificate
2000-11-01
2002-10-15
Gupta, Yogendra N. (Department: 1751)
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
Enzyme component of specific activity or source
C510S392000, C510S305000, C510S312000, C510S276000, C510S320000, C510S374000, C435S007100, C435S068100, C435S069100, C435S188000
Reexamination Certificate
active
06465410
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to laundry detergent and/or fabric care compositions comprising a modified protein which comprises a catalytically active amino acid sequence of an antimicrobial enzyme and/or an amino acid sequence of an antimicrobial peptide, linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD), for sanitisation benefits.
BACKGROUND OF THE INVENTION
Modern laundry detergent and/or fabric care compositions contain various detergent ingredients having one or more purposes in obtaining fabrics which are not only clean but also have retained appearance and integrity. Therefore, detergent components such as perfumes, soil release agents, fabric brightening agents, fabric softeners, chelants, bleaching agents and catalysts, dye fixatives and enzymes, have been incorporated in laundry detergent and/or fabric care compositions. One of such specific example is the use of enzymes, especially proteases, lipases, amylases and/or cellulases. The use of enzymes to remove stains comprising proteins and/or carbohydrates, in combination with various detergent, is well known in the art of detergent formulations.
Moreover, laundry detergent and/or fabric care composition having antimicrobial activities are of interest to consumers. Efforts to formulate antimicrobial hand soaps and cleaning compositions are well known. Efforts to produce laundry compositions comprising enzymes having microbial properties are also known. In spite of such efforts, there continues to be a need for laundry detergent and/or fabric care compositions having antimicrobial activity.
However, it has been difficult to incorporate enzymes into modern detergents in an effective manner. In that regard, those skilled in the art have sought to use minimal amounts of enzyme to their fullest effectiveness by ensuring that most, if not all, of the enzyme comprised in the detergent composition deposits on the fabric. For example, the optimum cellulase would have a binding domain especially suitable for cellulose. In this way, most of the cellulase enzyme included in the detergent composition deposits or otherwise binds to the fabric during the laundering cycle to achieve its desired results.
Similarly, it would be desirable to have laundry detergent and/or fabric care compositions in which its enzymatic and/or peptidic components are also modified to ensure deposition onto the fabrics for improved or new performances.
Accordingly, there remains a need for laundry detergent and/or fabric care compositions which provide improved sanitisation of the fabrics to be laundered. There also remains a need for such antimicrobial enzymes and/or antimicrobial peptide which are suitable for use in modern laundry detergent and/or fabric care compositions to be formulated in an effective manner.
The above objectives have been met by formulating laundry detergent and/or fabric care compositions comprising a modified protein which comprises a catalytically active amino acid sequence of an antimicrobial enzyme and/or an amino acid sequence of an antimicrobial peptide, linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD), for sanitisation benefits. It has been surprisingly found that said modified protein more readily attaches, affixes or otherwise comes into contact with the fabric, thereby resulting in increased or enhanced performance of the enzyme and/or antimicrobial peptide. In particular, the laundry detergent and/or fabric care compositions of the present invention when comprising proteins so modified, provide a higher effective concentration of the enzyme and/or antimicrobial peptide at its substrate location and therefore, improved sanitisation benefits.
Enzymes linked to Cellulose Binding Domains are also described in the art: WO91/10732 novel derivatives of cellulase enzymes combining a core region derived from an endoglucanase producible by a strain of Bacillus spp., NICMB 40250 with a CBD derived from another cellulase enzyme or a combining a core region derived from another cellulase enzyme with a CBD derived from said endoglucanase, for improved binding properties. WO94/07998 describes cellulase variants of a cellulase classified in family 45, comprising a CBD, a Catalytically Active Domain (CAD) and a region linking the CBD to the CAD, wherein one or more amino acid residues have been added, deleted or substituted and /or another CBD is added at the opposite end of the CAD. WO95116782 relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in
Trichoderma longibrachiatum
comprising different core regions with several CBDs. WO97/01629 describes cellulolytic enzyme preparation wherein the mobility of the cellulase component may be reduced by adsorption to an insoluble or soluble carrier e.g. via the existing or newly introduced CBD. WO97/28243 describes a process for removal or bleaching or soiling or stains from cellulosic fabrics wherein the fabric is contacted in aqueous medium with a modified enzyme which comprises a catalytically active amino acid sequence of a non-cellulolytic enzyme selected from amylases, proteases, lipases, pectinases and oxidoreductases, linked to an amino acid sequence comprising a cellulose binding domain and a detergent composition comprising such modified enzyme and a surfactant.
U.S. Pat. No. 5,258,304 describes methods using Type II endoglycosidases alone or in combination with other enzymes, detergents, surfactants and/or disulfide cleaving reagents to facilitate the removal of micro-organisms such as bacteria from the surface of materials such as fabric, contact lenses, metal, ceramics, cell tissue and the like. A method for cleaning a surface on which is bound a glycoside-containing substance such as blood or components thereof, fecal matter or components thereof or micro-organisms is described in U.S. Pat. No. 5,238,843. U.S. Pat. No. 5,395,541 discloses cleaning compositions containing a first enzyme selected from Endo-D, Endo-H, Endo-L, Endo-C, Endo-CII, Endo-F-Gal type, Endo-F and/or PNGaseF; a second enzyme selected from protease, lipase, nuclease, glycosidase; a detergent surfactant and a builder. The substrates can be blood or components thereof, fecal matter or components thereof or microorganisms and the surfaces can be fabrics, biological tissue, tooth enamel, contact lens, glass, ceramic, metal, metal alloy, plastic, plant, fruit and vegetables. U.S. Pat. No. 5,356,803 describes an antimicrobial composition consisting essentially of a Type II endoglycosidase and antimicobial agent such as bactericide, fungicide and/or algicide, used in the form of personal care or household cleaning products such as laundry detergent. U.S. Pat. No. 5,041236 is directed to an antimicrobial composition comprising endo-&bgr;-N-acetylglucosaminidase and/or endoglycopeptidase and ruminant stomach lysozyme and a method for destroying or removing microbes by treatment with these enzymes.
U.S. patent application Ser. No. 60/045,756 filed Jun. 5, 1997 describes laundry or cleaning products comprising one or more hexosaminidase and methods for laundering fabrics and cleaning hard surfaces with an aqueous solution containing an effective amount of one or more hexosaminidase enzymes. U.S. patent application Ser. No. 601045,826 filed May 5, 1997 describes laundry or cleaning products comprising one or more enzymes exhibiting endoglucanase activity specific for xyloglucan and methods for laundering fabrics and cleaning hard surfaces with an aqueous solution containing an effective amount of one or more enzymes exhibiting endoglucanase activity specific for xyloglucan.
Patent application PCT/US97102534 discloses cleaning compositions comprising an endo-dextranase enzyme, which provide enhanced overall cleaning and sanitisation of the treated surfaces together with malodour control. Similarly, mycodextranases are described in the co-pending application PCT/US96/15572 filed on Sep. 27, 1996.
However, none of these documents disclose a laundry detergent and/or fabric care compositions
Bettiol Jean-Luc Philippe
Boyer Stanton Lane
Smets Johan
Cook C. Brant
Elhilo Eisa
Gupta Yogendra N.
Miller Steve W.
The Procter & Gamble
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