Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...
Reexamination Certificate
1999-01-22
2002-12-03
Delcotto, Gregory (Department: 1751)
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
For cleaning a specific substrate or removing a specific...
C510S308000, C510S309000, C510S310000, C510S392000, C510S393000, C510S530000, C134S025200, C008S137000, C008S111000, C435S209000
Reexamination Certificate
active
06489279
ABSTRACT:
TECHNICAL FIELD
The present invention relates to laundry and cleaning compositions comprising enzymes with xyloglucanase activity.
BACKGROUND OF THE INVENTION
As previously described in WO 94/14953, published Jul. 7, 1994 by Novo Nordisk, endoglucanases (EC no. 3.2.1.4) constitute a group of hydrolases which catalyze endo hydrolysis of 1,4-&bgr;-D-glycosidic linkages in cellulose, cellulose derivatives (such as carboxy methyl cellulose and hydroxy ethyl cellulose), lichenin, &bgr;-1,4 bonds in mixed &bgr;-1,3 glucans (such as cereal &bgr;-D-glucans or xyloglucans) and other plant material containing cellulosic parts. The authorized name is endo-1,4-&bgr;-D-glucan 4-glucano hydrolase, but the abbreviated term endoglucanase is also used.
Endoglucanases have been found to be produced by various types of organisms such as plants and microorganisms, and endoglucanases of a wide variety of specificities are said to have been identified. For instance, xyloglucan specific endoglucanases have been identified in various plants, see for example, the disclosure of Fry et al.,
Biochem. J
. (1992), Vol 282, pp 821-828, Nishitani and Tominaga,
The Journal of Biol. Chemistry
(1992). Vol. 267, No. 29, pp. 21058-21064, Hayashi et al.,
Plant Physiol
., (1984), Vol. 75, pp. 605-610, McDougall and Fry,
J. Plant Physiol
., (1991), Vol. 137, pp. 332-336, and WO 93/17101. All of these enzymes have been found to have transferase activity (as defined e.g. by Fry et al., 1992 and Nishitani et al., 1992) and are therefore said not to be classified as a real endoglucanase. Further, a xyloglucan specific endoglucanases in microorganisms is described in WO 94/14953. Therein, it is generally stated that “endoglucanases having a high xyloglucan-degrading activity may be of particular use for degradations of cell wall material having a high xyloglucan content, for instance in the wine and fruit industry, for pectin-extraction and for removal of hemicelluloses from textile fibres”. Specifically referred to for this last property is the use of these enzymes to manufacture textile fibers:
“The hemicellulose like xyloglucan has to be removed from plant fibers like cotton, flax, hemp and jute before these can be used for textiles. For this purpose endoglucanase of type II [i.e., the xyloglucan-specific enzymes] has the advantage that it specifically removes the xyloglucan without damaging the cellulose. This endoglucanase may be used alone or together with other enzymes (e.g. pectinases) active on the pectic substances on the fibers.”
“Furthermore, the endoglucanases of the invention and analogous thereof may be used to treat cellulose fibres or cellulose-fibre rich material. The endoglucanases may e.g. be used in the paper industry to improve the drainage of pulp, and to treat fabrics such as cotton fabrics, to give a more smooth fabric.” [At page 12.]
An object of the invention is to provide laundry and cleaning compositions containing enzymes with a specified level of xyloglucanase activity. These and other objects will be apparent from the detailed description herein.
BACKGROUND DOCUMENTS
See: Fry et al.,
Biochem. J
. (1992), Vol. 282, pp 821-828, Nishitani and Tominaga,
The Journal of Biol. Chemistry
(1992). Vol. 267, No. 29, pp. 21058-21064, Hayashi et al.,
Plant Physiol
., (1984), Vol. 75, pp. 605-610, McDougall and Fry,
J. Plant Physiol
., (1991), Vol. 137, pp. 332-336, WO 93/17101, and WO 94/14953. See also: US 5,356,803 for use of Type II endoglycosidases (Endo-D, Endo-H, Endo-F and PNGaseF) in laundry and cleaning compositions.
SUMMARY OF THE INVENTION
The present invention relates to laundry or cleaning products comprising one or more enzymes exhibiting endoglucanase activity specific for xyloglucan, preferably at a level of from about 0.001% to about 1%, more preferably from about 0.01% to about 0.5%, by weight of the composition. The present invention also relates to a method for laundering fabrics (preferably clothes), said method comprising contacting fabrics in need of cleaning with an aqueous solution containing an effective amount of one or more enzymes exhibiting endoglucanase activity specific for xyloglucan, preferably an aqueous solution of a composition according to the present invention. The present invention further relates to a method for cleaning dishes and tableware, said method comprising contacting dishes or tableware in need of cleaning with an aqueous solution containing an effective amount of one or more enzymes exhibiting endoglucanase activity specific for xyloglucan, preferably an aqueous solution of a composition according to the present invention, and more preferably in an automatic dishwashing machine.
As used herein, the term “endoglucanase activity” means the capability of the enzyme to hydrolyze 1,4-&bgr;-D-glycosidic linkages present in any cellulosic material, such as cellulose, cellulose derivatives, lichenin, &bgr;-D-glucan, or xyloglucan. The endoglucanase activity may be determined in accordance with methods known in the art, examples of which are described in WO 94/14953 and hereinafter. One unit of endoglucanase activity (e.g. CMCU, AVIU, XGU or BGU) is defined as the production of 1 &mgr;mol reducing sugar/min from a glucan substrate, the glucan substrate being, e.g., CMC (CMCU), acid swollen Avicell (AVIU), xyloglucan (XGU) or cereal &bgr;-glucan (BGU). The reducing sugars are determined as described in WO 94/14953 and hereinafter. The specific activity of an endoglucanase towards a substrate is defined as units/mg of protein.
More specifically, the invention relates to laundry and cleaning compositions comprising an enzyme exhibiting as its highest activity XGU endoglucanase activity (hereinafter “specific for xyloglucan”), which enzyme:
i) is encoded by a DNA sequence comprising or included in at least one of the following partial sequences
(a) ATTCATTTGT GGACAGTGGA C (SEQ ID No: 1)
(b) GTTGATCGCA CATTGAACCA (SEQ ID NO: 2)
(c) ACCCCAGCCG ACCGATTGTC (SEQ ID NO: 3)
(d) CTTCCTTACC TCACCATCAT (SEQ ID NO: 4)
(e) TTAACATCTT TTCACCATGA (SEQ ID NO: 5)
(f) AGCTTTCCCT TCTCTCCCTT (SEQ ID) NO: 6)
(g) GCCACCCTGG CTTCCGCTGC CAGCCTCC (SEQ ID NO: 7)
(h) GACAGTAGCA ATCCAGCATT (SEQ ID NO: 8)
(i) AGCATCAGCC GCTTTGTACA (SEQ ID NO: 9)
(j) CCATGAAGTT CACCGTATTG (SEQ ID NO: 10)
(k) GCACTGCTTC TCTCCCAGGT (SEQ ID NO: 11)
(l) GTGGGCGGCC CCTCAGGCAA (SEQ ID NO: 12)
(m) ACGCTCCTCC AATTTTCTCT (SEQ ID NO: 13)
(n) GGCTGGTAG TAATGAGTCT (SEQ ID NO: 14)
(o) GGCGCAGAGT TTGGCCAGGC (SEQ ID NO: 15)
(p) CAACATCCCC GGTGTTCTGG G (SEQ ID NO: 16)
(q) AAAGATTCAT TTGTGGACAG TGGACGTTGA TCGCACATTG AACCAACCCC AGCCGACCGA
TTGTCCTTCC TTACCTCACC ATCATTTAAC ATCTTTCAC CATGAAGCTT TCCCTTCTCT
CCCTTGCCAC CCTGGCTTCC GCTGCCAGCC TCCAGCGCCG CACACTTCTG CGGTCAGTGG
GATACCGCCA CCGCCGGTGA CTTCACCCTG TACAACGACC TTTGGGGCGA GACGGCCGGC
ACCGGCTCCC AGTGCACTGG AGTCGACTCC TACAGCGGCG ACACCATCGC TTGTCACACC
AGCAGGTCCT GGTCGGAGTA GCAGCAGCGT CAAGAGCTAT GCCAACG (SEQ ID NO:17) or
(r) CAGCATCTCC ATTGAGTAAT CACGTTGGTG TTCGGTGGCC CGCCGTGTTG CGTGGCGGAG
GCTGCCGGGA GACGGGTGGG GATGGTGGTG GGAGAGAATG TAGGGCGCCG TGTTTCAGTC
CCTAGGCAGG ATACCGGAAA ACCGTGTGGT AGGAGGTTTA TAGGTTTCCA GGAGACGCTG
TATAGGGGAT AAATGAGATT GAATGGTGGC CACACTCAAA CCAACCAGGT CCTGTACATA
CAATGCATAT ACCAATTATA CCTACCAAAA AAAAAAAAAA AAAAAAAAAA AAAA (SEQ ID NO: 18)
or a sequence homologous thereto encoding a polypeptide specific for xyloglucan with endoglucanase activity,
ii) is immunologically reactive with an antibody raised against a highly purified endoglucanase encoded by the DNA sequence defined in i) and derived from
Aspergillus aculeatus
, CBS 101.43, and is specific for xyloglucan.
More specifically, as used herein the term “specific for xyloglucan” means that the endoglucanse enzyme exhibits its highest endoglucanase activity on a xyloglucan substrate, and preferably less than 75% activity, more preferably less than 50% activity, most preferably less than about 25% activity, on other cellulose-containing substrates such as carboxymethyl cellulose, cellulose, or other glucans.
Preferably, the specificity of an endoglucanase towards xyloglucan is furt
Convents Andre Christian
Moese Rosa Laura
Cook C. Brant
Delcotto Gregory
Miller Steven W.
The Procter & Gamble & Company
Zerby Kim W.
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