Latex agglutination determination of IgG in neonatals and in col

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic

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436533, G01N 3354

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active

045421030

ABSTRACT:
A method for determining the amount of IgG present in a neonatal foal or calf or in the colostrum of a dam or cow. The method involves contacting a body fluid sample with biologically inert latex particles, measuring the amount of agglutination which occurs, and determining the amount of IgG present. The latex particles are diluted to a final concentration of from about 0.25 to 2.0 percent (w/v) at a pH of from about 7.5 to 9.0 with a buffer. The body fluid is diluted to a range of from 0.01:5 parts to 0.01:2160 parts, v/v basis, at a pH of from about 7.5 to 9.0.

REFERENCES:
patent: 3873683 (1975-03-01), Fishbein
patent: 3992517 (1976-11-01), Lowke
patent: 4003988 (1977-01-01), Hoff
Chemical Abstracts, 93: 69500a, (1980).

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