LAT1 transporters expressed in blood brain barrier cells

Details LAT1 transporters expressed in blood brain barrier cells LAT1 transporters expressed in blood brain barrier cells

2004-12-30

2008-12-09

Nickol, Gary B. (Department: 1646)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007200, C435S007300, C435S069100, C435S235100, C435S320100, C435S325000, C530S350000, C536S023100, C536S023500

Reexamination Certificate

active

07462459

ABSTRACT:
LAT1 is consistently expressed at high levels in brain microvessel endothelial cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is actively transported by, the LAT1 transporter, and therefore a candidate substrate for crossing the blood brain barrier. The assays are useful in screening for therapeutic, cytotoxic or imaging compounds used in the treatment or diagnosis of neurological diseases.

REFERENCES:
patent: 6489302 (2002-12-01), Wiessler et al.
patent: 2003/0158254 (2003-08-01), Zerangue et al.
Alderman, D.A.; “A Review of Cellulose Ethers in Hydrophilic Matrices for Oral Controlled-Release Dosage Forms”;Int. J. Pharm. Tech. &Prod. Mfr. 5(3):1-9 (1984).
Altschul, S. et al.; “Basic Local Alignment Search Tool”;J. Mol. Biol. 215:403-410 (1990).
Audus, K. et al.; Characterization of an in vitro blood-brain barrier model system for studying drug transport and metabolism,Pharmaceutical Res. 3(2):81-87 (1986)).
Audus, K. et al.; The use of cultured epithelial and endothelial cells for drug transport and metabolism studies,Pharmaceutical Res. 7(5):435-451 (1990)).
Bamba, M. et al.; “Release Mechanisms in Gelforming Sustained Release Preparations”;Int. J. Pharmaceuticals2:307-315 (1979).
Bowman, P. et al.; “Brain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeability”;Ann. Neurol. 14:396-402 (1983).
Brightman and Neuwelt (ed.); Implications of the blood-brain barrier and its manipulation; vol. 1, Plenum Medical, New York, pp. 53-83 (1989).
Cserr, H. et al.,; “Blood-brain interfaces in vertebrates: a comparative approach”;Am. J. Physiol. Regulatory, Integrative and Comparative Physiology246:277-288 (1984).
During, M. et al.; “Controlled Release of Dopamine from a Polymeric Brain Implant: In Vivo Characterization”;Ann. Neurol. 25:351-356 (1989).
Goldstein, G. et al.; “The Blood-Brain Barrier”;Scientific American255(3):74-83 (1986).
Hanes, J. et al.; “New Advances in Microsphere-Based Single-Dose Vaccines”;Advanced Drug Delivery Reviews28:97-119 (1997).
Henikoff, S. et al.; Amino Acid Substitution Matrices from Protein Blocks;Proc. Natl. Acad. Sci. USA89:10915-19 (1989).
Howard, M. et al.; “Intracerebral Drug Delivery in Rats with Lesion-Induced Memory Deficits”;J. Neurosurg. 71:105-112 (1898—).
Karlin, S. et al.; “Applications and Statistics for Multiple High-Scoring Segments in Molecular Sequences”;Proc. Nat'l. Acad. Sci. USA90:5873-87 (1993).
Langer, R.; “New Methods of Drug Delivery”;Science249:1527-1533 (1990).
Langer, R. et al.; “Chemical and Physical Structure of Polymers as Carriers for Controlled Release of Bioactive Agents: A Review”;J. Macromol. Sci. Rev. Macromol Chem. 23:61-126 (1983).
Levy, R. et al.; “Inhibition of Calcification of Bioprosthetic Heart Valves by Local Controlled-Release Diphosphonate”;Science228:190-193 (1985).
Masereeuw, R. et al.; “In vitro and in vivo transport of zidovudine (AZT) across the blood-brain barrier and the effect of transport inhibitors”;Pharm. Res. 11(2):324-330 (1994).
Meresse, S. et al.; “Bovine brain endothelial cells express tight junctions and monoamine oxidase activity in long-term culture”;J. Neuorchem. 53:1363-1371 (1989).
Needleman, S. et al.; “A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins”;J. Mol. Biol. 48:443-453 (1970).
Pardridge, W. M.; “Receptor-Mediated Peptide Transport Through the Blood-Brain Barrier”; (1986)Endocrine Rev. 7(3):314-330 (1986).
Pardridge, W. M. et al.; “Comparison of in vitro and in vivo models of drug transcytosis through the blood-brain barrier”;J. Pharmacol. Exp. Thera. 253(2):884-891 (1990).
Pearson, W. et al.; “Improved Tools for Biological Sequence Comparison”;Proc. Nat'l Acad. Sci. USA85:2444-48 (1988).
Smith, T. et al.; “Comparison of Biosequences”;Adv. Appl. Math. 2:482-489 (1981).
Terasaki, T. et al.; “New Approaches to in vitro Models of Blood-Brain Barrier Drug Transport”;Drug Discovery Today8:944-954 (2003).
Tamai et al., “Transporter-mediated permeation of drugs across the blood-brain barrier,”J. Pharmaceutical Sciences, 89(11):1371-1388 (2000).
Terasaki et al., “Conditionally immortalized cell lines as a new in vitro model for the study of barrier functions,”Biological and Pharmaceutical Bulletin, 24(2):111-118 (2001).

Affiliated with

Also associated with

Rating

LAT1 transporters expressed in blood brain barrier cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with LAT1 transporters expressed in blood brain barrier cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and LAT1 transporters expressed in blood brain barrier cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4045043