Laser microdissection device

Details Laser microdissection device Laser microdissection device

2006-04-25

2006-04-25

Robinson, Mark A. (Department: 2872)

Optical: systems and elements

Compound lens system

Microscope

C359S381000, C359S211200, C359S368000, C606S017000

Reexamination Certificate

active

07035004

ABSTRACT:
The invention relates to a laser microdissection device comprised of a microscope table (1), which supports a specimen (3) to be dissected, of an incident lighting device (7), a laser light source (5) and of an objective (10) for focussing the laser beam (18) of the laser light source (5) onto the specimen (3). According to the invention, the microscope table (1) is not moved during the dissecting process. A laser scanning device (9) is arranged in the incident lighting device (7), is comprised of two thick glass wedge plates (11a, 11b), which are tilted toward the optical axis (8) and can be rotated independently of one another around said optical axis (8). In addition to the beam deviation caused by the wedge angle of the wedge plates (11a,11b), a beam offset of the laser beam (18) is produced by the thickness and the tilt of the wedge plates (11a,11b). When both wedge plates (11a,11b) are rotated, the beam deviation and the beam offset of the laser beam (18) are varied in such a manner that the laser beam (18) always passes through the middle of the objective pupil (19) and, at the same time, the beam is guided over the specimen (3) to be dissected by the beam deviation of the laser beam (18).

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