Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1992-04-27
1993-08-24
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
422 8205, 422 8209, 435 723, 435 724, 435 732, 436526, 436805, 436806, 436813, 436817, 356337, C12Q 170, G01N 33553
Patent
active
052388115
ABSTRACT:
A laser magnetic immunoassay (LMIA) technique is presented which combines the high detection sensitivity of the radioimmunoassay (RIA) technique with the simplicity of particle agglutination (PA) technique. The LMIA technique dispenses with the separation step of bound and free species (B/F separation), yet provides detectivity of the order of picogram of viral species per milliliter of analyte solution. The procedure includes of the steps of: preparing a superparamagnetic-labeled body of antigen or antibody; subjecting a specimen sample and the magnetic-labeled body to a specific immunoreaction to produce a reacted body; subjecting the reacted body containing both bound and free species to a spot magnetic field gradient; irradiating the spot with a laser beam; measuring the intensity of the outgoing light from the spot; and making a quantitative determination of the virus according to the time difference in the outgoing light signals generated by bound and free species.
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Arishima Koichi
Fujiwara Koichi
Hoshino Mitsutoshi
Hoshino Yasushi
Mizutani Hiroko
Kepplinger Esther L.
Nippon Telegraph and Telephone Corporation
Wolski Susan C.
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