Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-09-19
2000-03-21
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435287, C12Q 168, C12M 100
Patent
active
060401397
ABSTRACT:
A process and apparatus for cell purification and ablation is disclosed. The present invention comprises a laser system which directs radiant energy at computer or manually selected individual cells thereby disrupting DNA, RNA and protein structure in those cells. The present invention produces a purified tissue section containing relatively intact DNA, RNA or protein from only the untreated cells. This purified sample is suitable for amplification of material by PCR or other techniques for the analysis of molecular genetic features in the selected cells of interest.
REFERENCES:
patent: 4395397 (1983-07-01), Shapiro
patent: 4624915 (1986-11-01), Schindler et al.
patent: 4629687 (1986-12-01), Schindler et al.
patent: 4737628 (1988-04-01), Lovoi
patent: 4741043 (1988-04-01), Bacus
patent: 4785806 (1988-11-01), Deckelbaum
patent: 4785815 (1988-11-01), Cohen
patent: 4902132 (1990-02-01), Murphy, Jr. et al.
patent: 5098426 (1992-03-01), Sklar et al.
patent: 5158889 (1992-10-01), Hirako et al.
patent: 5272081 (1993-12-01), Weinreb et al.
patent: 5352613 (1994-10-01), Tafas et al.
patent: 5364744 (1994-11-01), Buican et al.
patent: 5374556 (1994-12-01), Bennett et al.
F. Klimek et al., Carcinogenesis, vol. 5, No. 2, pp. 265-268 (1984).
D. Koppel, Biophys. J., vol. 28, pp. 281-291 (Nov. 1979).
Y. Kubo et al., Cancer Research, vol. 55, pp. 989-990 (Mar. 1995).
D. Shibata et al., American Journal of Pathology, vol. 141, No. 3, pp. 539-543 (Sep. 1992).
Hadano et al., "Laser Microdissection and Single Unique Primer PCR Allow Generation of Regional Chromosome DNA CLones from a Single Human Chromosome," Genomics, vol. 11, pp. 364-373, 1991.
Marchevsky et al., "Computerized Interactive Morphometry as a Potentially Useful Tool in the Classification of Non-Hodgkin's Lymphomas," Cancer, vol. 57, pp. 1544-1549, Apr. 15, 1986.
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