Large scale separation and purification of fermentation product

Details Large scale separation and purification of fermentation product Large scale separation and purification of fermentation product

1994-10-20

1995-08-29

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 712, 435 713, 435183, 435187, 435198, 435272, 435800, 435814, 435815, 435816, 435874, C12N 930, C12N 900, C12P 2106

Patent

active

054459494

ABSTRACT:
A method of separating a hydrophobic fermentation product selected from the group consisting of lipase, esterase, endoxylanase and an antibiotic from a mixture comprising said product and contaminants which method comprises adding to the mixture sequentially
(1) 0.5 to 15% (w/v) of a nonionic surfactant,
(2) 0.5 to 60 mg of a flocculating agent per gram of said mixture,
(3) 1 to 20% (w/v) of an extra nonionic surfactant,
(4) a suitable K, Na, NH.sub.4 or Mg salt selected from the group of chlorides, sulfates, acetates, carbonates or phosphates, whereby the concentration of the salt is chosen so as to have the surfactant layer on top and is between 2 and 30%;
to obtain a three phase product mixture, separating the product mixture into liquid-liquid-solid fractions and recovering the hydrophobic fermentation product.

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