Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1973-12-20
1976-04-20
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
424 88, 424 89, 195 15, C07G 700
Patent
active
039519376
ABSTRACT:
Highly purified type B hepatitis antigen (HB Ag) is produced from fluid blood material containing such antigen by subjecting blood material containing naturally occurring HB Ag to a double precipitation with polyethylene glycol. In each precipitation, the pH of the fluid blood material is maintained at approximately 4.4 to 4.7 and a polyethylene glycol concentration of approximately 4.0 to 4.5 weight per cent is used. Particularly good results are obtained if the temperature of the material is maintained in the range of 0.degree. to 8.degree.C after the polyethylene glycol has been added. The antigen thus obtained may be further purified by absorption of impurities to hydroxy apatite, followed by isopycnic banding and zonal ultracentrifugation. Hydroxy apatite column chromatography is used to separate the purified antigen into three separate populations of particles.
REFERENCES:
patent: 3636191 (1972-01-01), Blumberg et al.
patent: 3790552 (1974-02-01), Johnson et al.
patent: 3838144 (1974-09-01), Leach
Laboratory Manual of Analytical Methods of Protein Chemistry, Alexander et al. 1960, pp. 70-72.
Prince Alfred M.
Vnek John
Schain Howard E.
The Community Blood Council of Greater New York, Inc.
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