Large scale production of DNA probes

Details Large scale production of DNA probes Large scale production of DNA probes

1984-11-27

1988-03-29

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 935 2, 935 78, 536 27, C12P 1934, C12Q 168

Patent

active

047343630

ABSTRACT:
A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide. The product can be used for making analytical probes.

REFERENCES:
patent: 4139346 (1979-02-01), Rabbani
patent: 4302204 (1981-11-01), Wahl
patent: 4486539 (1984-12-01), Ranki
patent: 4542102 (1985-09-01), Dattagupta
patent: 4563419 (1986-01-01), Ranki
Ashley, P. L. et al, Analytical Biochemistry, 140(1): 95-103 (1984), cited in Chem. Abst., CA 101(7): 509846y.
Microbiology, B. D. Davis et al, eds., Harper & Row, pub., Philadelphia (1980), pp. 171-178 and 207-210.

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