Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1989-03-27
1990-09-11
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435 25, 435147, 435168, 435188, 435190, 435259, 435803, 435815, 435816, 435938, C12N 904, C12N 902, C12R 184, C12Q 126
Patent
active
049562908
ABSTRACT:
A process for the purification of alcohol oxidase from whole cells of Pichia pastoris grown on methanol by the sequential steps of autolysis, crossflow filtration, ultrafiltration and recrystallization.
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Process Biochemistry, Apr. 1984, p. 67, Kroner, "Cross-Flow Filtration in the Downstream Processing of Enzymes".
ASM News, 50,299 (1984), Gabler, "Tangential Flow Filtration for Processing Cells, Proteins, and Other Biological Components".
Biotech & Bioengineering, 28,422 (1986), Devereux, "Membrane Separation of Protein Precipitates: Studies with Cross Flow in Hollow Fibers".
Enzyme Microb. Technol., 6,201 (1984), Quirk, "Investigation of the Parameters Affecting the Separation of Bacterial Enzyme from Cell Debris by Tangential Flow Filtration".
The Chemical Engineer, Jun. 1984, p. 10, Bertera, "Development Studies of Crossflow Microfiltration".
Biotechnology Letters, 7,471 (1985), Datar, "Studies on the Separation of Intracellular Soluble Enzymes from Bacterial Cell Debris by Tangential Flow Membrane Filtration".
Biotechnical Letters, 5,277 (1983), Quirk, "Tangential Flow Filtration A New Method for the Separation of Bacterial Enzymes from Cell Debris".
Harrison, Jr. Roger G.
Nelles Lynn P.
Lilling Herbert J.
Phillips Petroleum Company
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