Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1995-09-29
1997-08-26
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
435 6, 435 911, 536 243, 935 78, 935 85, 204450, 204456, 216 39, 423324, C12M 100, C12N 1500, B60R 900, C07H 2104
Patent
active
056610289
ABSTRACT:
A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.
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Micromachining a Miniaturized Capillary electrophoresis-Based chemical Analysis System on a Chip (Science Reports .circle-solid. vol. 261 .circle-solid. 13 Aug. 1993; pp. 895-897).
Lockheed Martin Energy Systems, Inc.
Sisson Bradley L.
Stafford Shelley L.
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