Large scale DNA microsequencing device

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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435 6, 435 911, 536 243, 935 78, 935 85, 204450, 204456, 216 39, 423324, C12M 100, C12N 1500, B60R 900, C07H 2104

Patent

active

056610289

ABSTRACT:
A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.

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patent: 5302509 (1994-04-01), Cheeseman
Micromachining a Miniaturized Capillary electrophoresis-Based chemical Analysis System on a Chip (Science Reports .circle-solid. vol. 261 .circle-solid. 13 Aug. 1993; pp. 895-897).

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