Optical: systems and elements – Compound lens system – Microscope
Patent
1996-07-18
1998-11-10
Nguyen, Thong
Optical: systems and elements
Compound lens system
Microscope
359363, 359368, G02B 2100, G02B 2136
Patent
active
058352651
ABSTRACT:
A microscope having a high effective numerical aperture (NA) is achieved in an apparatus in which a real, three-dimensional image is formed of an object placed in an aperture at the apex of one of two facing, axially-aligned concave mirrors. The three-dimensional image is acquired by a video camera positioned in a counterpart aperture at the apex of the other mirror and the acquired image is processed by computer. The processing corrects the acquired image using the point spread function of the mirror system which was previously obtained by positioning a point source of light throughout the object space and measuring the pixel values recorded by the camera's array of sensing element while the camera was positioned at different axial distances in the image space. When the diameter of the mirrors is large compared to the specimen object, substantially all of the light leaving the specimen is captured by the mirrors and focused upon the camera, thereby achieving a high effective numerical aperture for the system which affords excellent resolution, especially when examining transparent specimens.
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Computed Anatomy Incorporated
Nguyen Thong
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