Large DNA cloning system based on yeast artificial chromosomes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435255, 435256, 435320, 435912, 435 692, 435 94, 935 28, 935 55, 935 56, 935 67, C12N 1500, C12N 500, C12N 700

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048898066

ABSTRACT:
A large DNA cloning system is disclosed which is based on yeast artificial chromosomes. Cloning vectors are disclosed which allow the cloning of large segments of greater than 50 kb of exogenous DNA. The cloning vector comprises DNA sequences of an autonomous replication sequence, a centromere, a selectable yeast marker, two sequences that seed telomere function in vivo, and a cloning site within an interruptible yeast gene for insertion of the exogenous DNA segments.

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