Lambda packaging extract lacking beta-galactosidase activity

Details Lambda packaging extract lacking beta-galactosidase activity Lambda packaging extract lacking beta-galactosidase activity

1991-02-26

1993-02-23

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

43525233, 435 691, C12N 706, C12N 121

Patent

active

051889579

ABSTRACT:
A beta-galactosidase-free bacteriophage lambda DNA packaging extract is described. The extract can be produced from beta-galactosidase deficient lambda lysogens. Bacteriophage lambda lysogens having the genotype lacZ are provided.

REFERENCES:
Frischauf, from Methods in Enzymology vol. 152, 1987, pp. 190-212.
Maniatis et al, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 1st ed., pp. 504-505, 1982.
Miller, Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1972.
Hohn et al., Proc. Natl. Acad. Sci. USA, 74:3259-3263 (1977).
Maniatis et al., In Vitro Packaging of Bacteriophage .lambda. DNA, 256-268.
Rosenberg et al., Gene, 38:165-175 (1985).

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