Lambda integrase mediated recombination in plants

Details Lambda integrase mediated recombination in plants Lambda integrase mediated recombination in plants

2008-04-01

2008-04-01

Fox, David T. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S287000, C435S412000, C435S456000, C435S462000, C435S468000, C435S469000, C435S470000, C536S023720

Reexamination Certificate

active

10403232

ABSTRACT:
The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DNA within a plant cell, comprising: introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site; introducing into the plant cell a donor nucleotide sequence comprising a second Int recognition site; and introducing into the plant cell an integrase or integrase complex.

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