Lactoferricin gene and transformant expressing lactoferricin

Details Lactoferricin gene and transformant expressing lactoferricin Lactoferricin gene and transformant expressing lactoferricin

2007-03-06

2007-03-06

Weber, Jon (Department: 1653)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

C435S440000, C435S069100, C435S320100, C435S252300, C536S023100, C536S023500, C536S023400

Reexamination Certificate

active

10496806

ABSTRACT:
Disclosed is a gene encoding a mutated lactoferricin peptide and anE. colicell transformed with the same, wherein the mutated lactoferricin peptide has substitutions at the 7th and 10th amino acid residues of the wildtype bovine lactoferricin. The gene of the present invention can be expressed inE. coliat high efficiency, and the expressed mutated lactoferricin peptide can be purified at high yield, thus making its mass production possible.

REFERENCES:
patent: 5317084 (1994-05-01), Tomita et al.
patent: 6183992 (2001-02-01), Kim et al.
patent: WO 98/06860 (1998-02-01), None
patent: WO 00/12542 (2000-03-01), None
Vernooij et al. “Plant optimized Lactoferricin B constructing oligo 2”, SEQ ID No. 3, alignment result 4, Database: N—Geneseq—21, Accession No. AAV28423, Feb. 19, 1998.
Genbank Accession No. 1LFL, 'Bovine lactoferricin(Lfeinb) , Nmr, 20 Structures, Jun. 24, 1998.
Miyauchi et al., In: Cellular Immunology, 1998, vol. 187, pp. 34-37.

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