Lactobacilli harboring aggregation gene as a vaccine delivery ve

Details Lactobacilli harboring aggregation gene as a vaccine delivery ve Lactobacilli harboring aggregation gene as a vaccine delivery ve

1998-03-16

2000-08-08

Minnifield, Nita

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 231, 536 234, 536 2341, C07H 2104, C07H 2102, C07H 2100

Patent

active

061003883

ABSTRACT:
Live vaccines and methods for preparing the vaccines useful in protecting a host from infection by a pathogenic microorganism are provided. Vaccines are prepared from live Lactobacillus cells which have been transformed using DNA technology to express heterologous antigens of pathogenic microorganisms or other suitable biological material. Genes encoding antigenic determinants pathogenic in the mammalian gastrointestinal tract are inserted into expression cassettes and fused with genes encoding an aggregation factor and/or a mucin binding factor. The inserted genes are shown to transform L. reuteri cells. The aggregation enhancing and mucin binding genes have been isolated and sequenced. The vaccine can be ingested orally in a pharmaceutical carrier or in milk products.

REFERENCES:
patent: 5534253 (1996-07-01), Casas et al.
patent: 5837238 (1998-11-01), Casas et al.
patent: 5851794 (1998-12-01), Guss et al.
van der Lelie et al. Applied & Environmental Microbiology 57/1: 201-206, Jan. 1991.
Roos et al. FEMS Microbiol. Lett. 144:33-38, 1996.
Takahashi et al. Biosci. Biotech. Biochem. 60/9:1434-1438, 1996.
Patti et al. JBC, 267/7:4766-4772, 1992.
Jonsson et al. BBA 1249:65-71, 1995.
Kmet et al., FEMS Immunol. & Med. Microbiol., 19:111-114, 1997.
Roos, et al. "Autoaggregation of Lactobacillus reuteri is mediated by a putative DEAD-box helicase," XP002117044, Chemical Abstracts, abstract No. 85226, vol. 131 1999, and Mol. Microbiol. vol. 32, No. 2, 1999 pp. 427-436.
Wells, et al. "Lactic Acid Bacteria as vaccine delivery vehicles," Antonie Van Leeuwenhoek, vol. 70, 1996, pp. 317-3330, XP002117043.

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