Lactic bacteria producing exopolysaccharides

Details Lactic bacteria producing exopolysaccharides Lactic bacteria producing exopolysaccharides

1996-11-14

1998-07-28

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435183, 435193, 4352521, 4352523, 43525233, 4352525, 4352529, 4353201, 536 232, 536 237, C12P 1904

Patent

active

057861846

ABSTRACT:
DNA fragment of genomic origin coding for at least one enzyme involved in the biosynthesis of an EPS, and capable, following the transformation of a lactic bacteria, of restoring the production of an EPS in the said bacterium not initially producing any EPS, or of modifying the structure of the EPS initially produced by the said bacterium. Proteins of the Streptococcus thermophilus strain CNCM I-1590 encoded by the chromosome and which are involved in the biosynthesis of the EPS having the composition Glc:Gal:GalNac=1:2:1. Method for the manufacture of a new EPS, in which a DNA fragment coding partially or totally for at least one enzyme involved in the biosynthesis of an EPS is cloned into a vector, lactic bacteria producing another EPS are transformed with the recombinant vector, and a lactic bacterium producing a new EPS is then selected.

REFERENCES:
Rubens et al. Identification of cpsD, a gene essential for type III capsule expression in group B streptococci Mol. Microbiol, 8(5), 843-855, 1993.
Creighton, T. E. in "Proteins:Structure and Molecular Properties" Second Edition, W.H. Freeman and Company, New York, pp. 108, 109, 132 and 133, 1993.
Angelo Guidolin et al., "Nucleotide Sequence Analysis of Genes Essential for Capsular Polysaccharide Biosynthesis in Streptococcus Pneumoniae Type 19F", Infection and Immunity, vol. 62, No. 12, Dec. 1994, pp. 5384-5396.
Ernesto Garcis et al., "Cloning and Sequencing of a Gene involved in the Synthesis of the Capsular Polysaccharide of Streptococcus Phenumoniae Type 3" Molecular and General Genetics, vol. 239, No. 1-2, May 1993 Berlin de, pp 188-195.
Marisa Vescovo et al. , "Plasmid-Encoded Ropiness Production in Lactobacillus Casei SSP. Casei" Biotechnology Letter, vol. 11 No. 10, Oct. 1989, pp. 709-712.
Thierry Doco et al., "Structure of an Exocellular Polysaccharide Produced by Streptococucu Thermophilus", Carbohydrate Research, vol. 198, No. 2, May 1, 1990 Amsterdam NL, pp. 313-321.

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