Lactate dehydrogenase determination method

Details Lactate dehydrogenase determination method Lactate dehydrogenase determination method

1975-12-29

1977-02-01

Monacell, A. Louis

Textiles: fiber preparation

204195B, C12K 104

Patent

active

040060616

ABSTRACT:
A method of determining lactate dehydrogenase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing lactate, nicotinamide adenine dinucleotide and an electron transfer agent, and measuring the uptake of oxygen by the oxidation of the resulting NADH with an oxygen-sensing electrode.

REFERENCES:
patent: 3413198 (1968-11-01), Deutsch
patent: 3732147 (1973-05-01), Fosker et al.
patent: 3791931 (1974-02-01), Thum et al.
Ells, "A Colorimetric Method for the Assay of Soluble Succinic Dehydrogenase and Pyridinenucleotide-Linked Dehydrogenases," Archives of Biochem. & Biophy. vol. 85, pp. 561-562, (1959).
Amador et al., "Serum Lactic Dehydrogenase Activity: An Analytical Assessment of Current Assays," Clin. Chem., vol. 9, 1963, pp. 391-399.
Greenbaum et al., "The Estimation of the Oxidized and Reduced Forms of the Nicotinamide Nucleotides," Biochem. J., vol. 95, (1965), pp. 161-166.
Olson, "Differential Amperometric Measurement of Serum Lactate Dehydrogenase Activity Using Bindschedler's Green," Analytical Chem., vol. 46, No. 11, pp. 1544-1547, (1974).

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