Lactase preparation

Details Lactase preparation Lactase preparation

1980-03-24

1982-05-11

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435814, 435911, 435921, C12N 938

Patent

active

043294294

ABSTRACT:
A process for the release of lactase from yeast cells comprising contacting the cells with an alkyl alcohol or a dialkyl ketone, followed by extraction of the lactase in an aqueous solution at a pH in the range 5.5 to 8.0 is disclosed. Also disclosed is a process for the selective reduction of protease activity in lactase preparations comprising heating an aqueous solution of lactase containing protease impurities in the presence of glycerol. Lactase stability during the heating process can optionally be enhanced by addition of manganous ion.

REFERENCES:
patent: 2715601 (1955-08-01), Morgan
patent: 3592739 (1971-07-01), Sternberg
patent: 3885050 (1975-05-01), Ridgway et al.
patent: 3961080 (1976-06-01), Sugimoto et al.
patent: 4235970 (1980-11-01), Leach et al.
Yasumatsu et al., Agr. Biol. Chem.; vol. 29, No. 7, pp. 665 to 671, (1965).

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