Lactam-containing compositions and methods useful for the hybrid

Details Lactam-containing compositions and methods useful for the hybrid Lactam-containing compositions and methods useful for the hybrid

1990-07-27

1992-04-21

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

548543, 548546, 548547, 546243, 540485, 540526, 540527, 540451, 540463, C12Q 168

Patent

active

051067307

ABSTRACT:
This invention relates to novel methods for the release of nucleic acids from cells in complex biological samples or specimens to prepare and make available the nucleic acid material present for a hybridization assay or for extraction. Novel methods for hybridization of nucleic acids are also presented. In particular methods are described for isolating nucleic acid from a sample containing a complex biological mixture of nucleic acid and non-nucleic acids wherein the sample is combined with a hybridization medium comprising a lactam which promotes and enables nucleic acid pairing when complementary nucleic acid is introduced. The lactam is preferably about 5 to about 70% of the hybridization medium and is most preferably 2-pyrrolidone, N-ethyl-2-pyrrolidone, N-cyclohexyl-2-pyrrolidone, N-dodecyl-2-pyrrolidone, N-methyl-2-pyrrolidone, N-hydroxyethyl-2-pyrrolidone, N-methyl-2-piperidone, 2-.epsilon.-caprolactam, N-methyl-2-caprolactam, 2-piperidone or N-(4-hydroxybenzyl)pyrrolidone.

REFERENCES:
patent: 4302204 (1981-11-01), Wahl
patent: 4483920 (1984-11-01), Gillespie
patent: 4486539 (1984-12-01), Ranki
patent: 4563419 (1986-01-01), Ranki
Marmer, J. Mol. Biol. vol. 3, pp. 208-218 (1961).
Chirgwin et al., Biochem vol. 18, pp. 5294-5299 (1979).
Dunn et al., Cell vol. 12, pp. 23-36 (1977).
Thompson et al., Anal. Biochem. vol. 163, pp. 281-291 (1987).
Bresser et al., DNA vol. 2, pp. 243-254 (1983).
Manser et al., Proc. Natl. Acad. Sci. U.S.A. vol. 81, pp. 2470-2474 (1984).

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