Lac shuttle vectors

Details Lac shuttle vectors Lac shuttle vectors

2006-04-11

2006-04-11

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Vector, per se

C435S004000, C435S006120, C435S014000, C435S007370, C424S093210, C514S04400A, C536S023100

Reexamination Certificate

active

07026162

ABSTRACT:
The invention discloses a Lac shuttle vector, comprising at least (a) a region which regulates a plasmid copy number; (b) an eukaryotic gene expression cassette, which comprises at least an eukaryotic gene transcriptional promoter sequence, a multiple cloning site and a transcriptional terminator sequence; (c) a lactic acid bacteria plasmid sequence, which comprises a plus origin of replication, and a nucleic acid sequence encoding for a protein which relates to the lactic acid bacteria plasmid replication; and (d) a non-antibiotic resistance selection gene and the promoter sequence thereof. The Lac shuttle vector features a non-antibiotic resistance gene as a selection marker, which is useful in pharmaceuticals and foods.

REFERENCES:
Ertl et.al.; Genetic Immunization, 1996, Viral Immunology vol. 9, No. 1: 1-9.
Ross et.al.; Gene Therapy in the United States: A five-Year Status Report, 1996, Human Gene Therapy 7: 1781-1790.
Fox; No winners against AIDS, 1994, Bio/Technology vol. 12: 128.
Yasutomi; A Vaccine-Elicited, Single Viral Epitope- specificSpecific CytotoxicTLymphocyte . . . Simian Immunodeficiency Virus Challenge, 1995, Journal of Virology: 2279-2284.
Monteil et.al.; Genetic Immunization of seronegative one-day-old piglets against pseudorabies induces neutralizing antibodies but not protection and is ineffective in piglets from Immune dams, 1996, Elsevier 27: 443-452.
Orkins et.al.; Report and Recommendations of the Panel to Assess the NIH Investment in Research on Gene Therapy, 1995.
Bringel et.al.; Characterization, Cloning, Curing, and Distribution in Lactic Acid Bacteria of pLP1, a Plasmid . . . Its use in Shuttle Vector Construction, 1989, Plasmid 22: 193-202.
Hemme et.al.; Expression ofLactobacillus caseiATCC 393 . . . inLactococcus lactisCNRZ . . . 1123, 1994, Letters in Applied Microbiology 19: 345-348.
Dietrich et.al; Delivery of antigen-encoding plasmid DNA into the cytosol of macrophages by attenuated suicideListeria monocytogenes, 1998, Nature Biotechnology, vol. 18: 181-185.
de Vos W.M. Safe and sustainable systems for food-grade fermentations by genetically modified lactic acid bacteria. International Dairy Journal, (1999) vol. 9, No. 1, pp. 3-10.
Cocconcelli et al. Genetic analysis of the replication region of theLactobacillusplasmid vector□□pPSC22. Res Microbiol. Oct. 1996;147(8):619-24.
Kanatani et al. Identification of the replication region ofLactobacillus acidphilusplasmid□□pLA103. FEMS Microbiol Lett. Nov. 1,1995;133(1-2):127-30.
Klein et al. Characterization and sequence analysis of a small cryptic plasmid from□□Lactobacillus curvatusLTH683 and its use for construction of newLactobacillus□□cloning vectors. Plasmid. Jul. 1993;30(1):14-29.
Imanaka et al. Complete nucleotide sequence of the low copy number plasmid pRAT11 and □□replication control by the RepA protein inBacillus subtilis. Mol Gen Genet. Oct. 1986;205(1):90-6.
Sorensen et al. A food-grade cloning system for industrial strains ofLactococcus lactis.□□Appl Environ Microbiol. Apr. 2000;66(4):1253-8.
Platteeuw et al. Food-grade cloning and expression system forLactococcus lactis.□□Appl Environ Microbiol. Mar. 1996;62(3):1008-13.
Bouia et al. Structural organization of pLP1, a cryptic plasmid fromLactobacillus planatarumCCM 1904. Plasmid. Nov. 1989;22(3):185-92.

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