Labelling of oligonucleotides

Details Labelling of oligonucleotides Labelling of oligonucleotides

1985-03-15

1989-02-28

Warren, Charles F.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 78, 935 8, 435 91, C12Q 168, C12P 1934

Patent

active

048085200

ABSTRACT:
A method is provided of making an oligonucleotide which comprises hybridizing (a) a shorter fragment of the desired oligonucleotide with (b) a nucleic acid fragment longer than (a) and complementary to the desired oligonucleotide, one of (a) and (b) is linked to an organic radical which differentiates its physical properties relative to the other of (a) and (b), contacting the hybridized material with an enzyme and nucleoside triphosphates whereby the shorter fragment is extended in one direction until it is substantially coterminal with the complementary nucleic acid fragment, denaturing the hybridized material and separating lengthened (a) from (b).

REFERENCES:
Tener, G. M. et al., Biochemistry, vol. 17, No. 4, 1978, pp. 741-745.
Dattagupta, N. et al., Biotechniques, vol. 5, No. 1, 1987, pp. 38, 39, 42, 43.
Meinkoth, J. et al., Anal Biochem., vol. 138, No. 2, 1984, pp. 267-269.
Kennell, D., In Progress in Nucleic Acid Research and Molecular Biology (Davidson, J. N. et al-ed), vol. 11, 1971, Academic Press, N.Y., pp. 259-261.
Studencki, A. et al., DNA, vol. 3, Feb. 1984, pp. 7-15.
Chem. Abs., vol. 101, No. 15, Oct. 8, 1984, p. 69, abst. no. 123160p, Svoboda, M. et al., "One-Step Isocratic . . . Cholecystokinin".
Randerath, K., Anal. Biochem, vol. 115, 1981, pp. 391-397.
Gene, vol. 33, 1985, pp. 191-196, Elseyier Science Publishers, New York, U.S.; K. M. Lang et al.: "Cloning Specific Complete Polyadenylylated 3'-Terminal cDNA Segments".

Affiliated with

Also associated with

Rating

Labelling of oligonucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Labelling of oligonucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Labelling of oligonucleotides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1366590