Labelling color, method of preparing the same and method for det

Details Labelling color, method of preparing the same and method for det Labelling color, method of preparing the same and method for det

1994-09-16

1996-01-30

McKane, Joseph K.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C07D20904

Patent

active

054881145

ABSTRACT:
A labelling color of pentamethine, its intermediate and a method for preparing and using the same to detect methamphetamine with high sensitivity.
The invention is exemplified by pentamethine of the following Formula (1): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 each are a C.sub.1-6 alkyl group, and X is an anion.
A method for using the labelling color to detect methamphetamine. The method comprises preparing a solution of the labelling color and methamphetamine-antibody in a buffer, measuring its fluorescent intensity at 660 nm in a 600 nm excited state, adding methamphetamine in a buffer to the solution, measuring its fluorescent intensity similarly, and examining the change in fluorescent intensity between both solutions.

REFERENCES:
patent: 3619239 (1971-11-01), Osada et al.
patent: 4425355 (1984-01-01), Hoefle et al.
patent: 4952336 (1990-08-01), Brynes et al.
patent: 4981977 (1991-01-01), Southwick et al.
patent: 5135863 (1992-08-01), Hu et al.
patent: 5378634 (1995-01-01), Sigetoh et al.
CA 120:293593s Pentamethinecyanine . . . methamphetamine. Shigefuji et al., p. 465, 1994.

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