Labeling reagents, methods for synthesizing such reagents...

Details Labeling reagents, methods for synthesizing such reagents... Labeling reagents, methods for synthesizing such reagents...

2002-05-03

2008-03-04

Gakh, Yelena G. (Department: 1797)

Chemistry: analytical and immunological testing

Tracers or tags

C436S063000, C436S501000, C436S544000, C436S546000, C435S006120, C536S023100

Reexamination Certificate

active

07338805

ABSTRACT:
The present invention relates to a temperature-stable labeling reagent of formula:in whichR1represents H or an alkyl, aryl or substituted aryl group,R2represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure,L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1,R3and R4represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR with R=alkyl or aryl,A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and—Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—.The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group.The invention finds a preferred application in the field of diagnosis.

REFERENCES:
patent: 4507466 (1985-03-01), Tomalia et al.
patent: 4568737 (1986-02-01), Tomalia et al.
patent: 4672040 (1987-06-01), Josephson
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4775745 (1988-10-01), Ford et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5234809 (1993-08-01), Boom et al.
patent: 5328824 (1994-07-01), Ward et al.
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5449767 (1995-09-01), Ward et al.
patent: 5750338 (1998-05-01), Collins et al.
patent: 6083708 (2000-07-01), Singh et al.
patent: 6083762 (2000-07-01), Papen et al.
patent: 6083763 (2000-07-01), Balch
patent: 6110426 (2000-08-01), Shalon et al.
patent: 39 10 151 (1990-10-01), None
patent: 0 063 879 (1982-11-01), None
patent: 0 097 373 (1984-01-01), None
patent: 0 201 184 (1986-12-01), None
patent: 0 286 898 (1988-10-01), None
patent: 0 302 175 (1989-02-01), None
patent: 0 350 407 (1990-01-01), None
patent: 0 561 722 (1993-09-01), None
patent: 0 567 841 (1993-11-01), None
patent: 0 569 272 (1998-06-01), None
patent: WO 90/01069 (1990-02-01), None
patent: WO 90/06995 (1990-06-01), None
patent: WO 90/08838 (1990-08-01), None
patent: WO 91/02818 (1991-03-01), None
patent: WO 93/16094 (1993-08-01), None
patent: WO 95/08000 (1995-03-01), None
patent: WO 97/35031 (1997-09-01), None
patent: WO 97/45202 (1997-12-01), None
patent: WO 98/05766 (1998-02-01), None
patent: WO 99/15621 (1999-04-01), None
patent: WO 99/35500 (1999-07-01), None
patent: WO 99/53304 (1999-10-01), None
patent: WO 99/65926 (1999-12-01), None
patent: WO 00/05338 (2000-02-01), None
patent: WO 00/07982 (2000-02-01), None
patent: WO 00/40590 (2000-07-01), None
patent: WO 00/60049 (2000-10-01), None
patent: WO 01/44506 (2001-06-01), None
patent: WO 01/92361 (2001-12-01), None
Shiga et al. “Diazonium Compounds Useful as Components for Nucleic Acid Probes”, Anal. Sci., 1993, v. 9, No. 4, pp. 553-556.
M. Shiga et al., “Fluorescence Detection of DNA Using A Novel Peroxidase Substrate, 4-(4-Hydroxyphenylcarbamoyl)butanoic Acid,”Analytical Sciences, vol. 11, No. 4, 1995, pp. 591-595.
G. M. Makrigiorgos et al., “Fluorescent Labelling of Abasic Sites: A Novel Methodology to Detect Closely-Spaced Damage Sites in DNA,”International Journal of Radiation Biology, vol. 74, No. 1, 1998, pp. 99-109.

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