Labeling reagents, methods for synthesizing such reagents...

Details Labeling reagents, methods for synthesizing such reagents... Labeling reagents, methods for synthesizing such reagents...

2008-03-04

2008-03-04

Gakh, Yelena G. (Department: 1797)

Chemistry: analytical and immunological testing

Tracers or tags

C436S063000, C436S501000, C436S544000, C436S546000, C435S006120, C536S023100

Reexamination Certificate

active

10137454

ABSTRACT:
The present invention relates to a temperature-stable labeling reagent of formula:in whichR1represents H or an alkyl, aryl or substituted aryl group,R2represents a detectable marker or at least two detectable markers linked together by at least one multimeric structure,L is a linking arm containing a linear succession of at least two covalent bonds and n an integer equal to 0 or 1,R3and R4represent independently of each other: H, NO2, Cl, Br, F, I, R2-(L)n-Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR with R=alkyl or aryl,A is a linking arm containing at least one covalent double bond allowing conjugation of the diazo functional group with the aromatic ring and u is an integer between 0 and 2, preferably equal to 0 or 1, and—Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—.The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group.The invention finds a preferred application in the field of diagnosis.

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