Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-11-27
1997-12-23
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 536 243, 435 6, C12Q 168, C07H 2102, C07H 1900
Patent
active
057009210
ABSTRACT:
Reagents and methods for multi-step labeling of nucleic acids allow the addition of relatively insoluble or unstable labels to nucleic acid in the final step. Nucleic acids can be stored as a stable intermediate capable of reacting with a label conjugated to a thiol-reactive group.
REFERENCES:
patent: 4487838 (1984-12-01), Hynes et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4737454 (1988-04-01), Dattagupta et al.
patent: 4822731 (1989-04-01), Watson et al.
patent: 4828979 (1989-05-01), Klevan et al.
patent: 4898951 (1990-02-01), Symons
patent: 4962029 (1990-10-01), Levenson et al.
patent: 5047519 (1991-09-01), Hobbs, Jr. et al.
M. Wilchek and E.A. Bayer, "Introduction to Avidin-Biotin Technology", and Applications of Avidin-Biotin Technology: Literature Survey, In: Methods in Enzymology, (Wilchek and Bayer, eds.), vol. 184, (1990).
L. Klevan and Gulilat Gebeyehu, "Biotinylated Nucleotides for Labeling and Detecting DNA," In: Methods in Enzymology (Wilchek and Bayer, eds.), vol. 184, pp. 561-577 (1990).
L. Angerer et al., "An Electron Microscope Study of the Relative Positions of the 4S and Ribosomal RNA Genes in HeLa Cell Mitochondrial DNA," Cell 9, pp. 81-90, (1976).
T. Kempe et al., "Chemical and Enzymatic Biotin-Labeling of Oligodeoxyribonucleotides," Nucleic Acids Res. 13 pp. 45-57, (1985).
B.C.F. Chu and L.E. Orgel, "Laboratory Methods: Detection of Specific DNA Sequences With Short Biotin-Labeled Probes," DNA 4, pp. 327-331 (1985).
S.Y. Cheng et al., "A Versatile Method for the Coupling Protein to DNA: Synthesis of a Macroglobulin-DNA Conjugates," Nucleic Acids Res. 11, pp. 659-669 (1983).
B.A. Connolly, "Chemical Synthesis of Oligonucleotides Containing a Free Sulphydryl Group and Subsequent Attachment of Thiol Specific Probes," Nucleic Acids Res. 13, pp. 4485-4502, (1985).
R. Zuckermann et al., "Efficient Methods for Attachment of Thiol Specific Probes to the 3'--Ends of Synthetic Oligodeoxyribonucleotides," Nucleic Acids Res. 15, pp. 5305-5321, (1987).
B.C.F. Chu and L.E. Orgel, "Ligation of Oligonucleotides to Nucleic Acids or Proteins Via Disulfide Bonds," Nucleic Acids Res. 16, pp. 3671-3691, (1988).
M. Shimkus et al., "A Chemically Cleavable Biotinylated Nucleotide: Usefulness in the Recovery of Protein-DNA Complexes from Avidin Affinity Columns," Proc. Natl. Acad. Sci. USA 82, pp. 2593-2597, (1985).
A. Kallioniemi et al., "Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors," Science 258, pp. 818-821, (1992).
T. Ried et al., "Simultaneous Visualization of Seven Different DNA Probes by in situ Hybridization Using Combinatorial Fluorescence and Digital Imaging Microscopy," Proc. Natl. Acad. Sci USA 89, pp. 1388-1392, (1992).
C. Kessler, "Review: Non-Radioactive Analysis of Biomolecules," Biotechnol. 35, pp. 165-189, (1994); and.
J.G.J. Bauman et al., "Cytochemical Hybridisation with Fluorochrome--Labelled RNA," Histochemistry 73, pp. 181-193, (1981).
Matthews et al., Anal. Biochemistry 169: 1--25 (1988).
Daniel Steven G.
Westling Mark E.
Jones W. Gary
Vector Laboratories
Whisenant Ethan
LandOfFree
Labeling nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Labeling nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Labeling nucleic acids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1803622