Labeling by simultaneous ligation and restriction

Details Labeling by simultaneous ligation and restriction Labeling by simultaneous ligation and restriction

1988-01-26

1992-03-03

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 35, 435810, 536 27, C12P 1934, C12Q 168, C12Q 116, C07H 1512

Patent

active

050932454

ABSTRACT:
Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.

REFERENCES:
patent: 4775619 (1988-10-01), Urdea
Ann-Christine Syvanen et al., "Fast Quantification of Nucleic Acid Hybrids by Affinity-based Hybrid Collection", Nuc. Acids Res. (1986) 14(12):5037-5048.
H. B. Gamper et al., "Reverse Southern Hybridization", Nuc. Acids Res. (1986) 14(24):9943-9954.
T. Goldkorn and D. J. Prockop, "A Simple and Efficient Enzymatic Method for Covalent Attachment of DNA to Cellulose, Application for Hybridization-Restriction Analysis and for in vitro Synthesis of DNA Probes", Nuc. Acids Res. (1986) 14(22):9171-9191.
A. C. Forster et al., "Non-radioactive Hybridization Probes Prepared by the Chemical Labelling of DNA and RNA with a Novel Reagent, Photobiotin", Nuc. Acids Res. (1985) 13(3):745-761.
J. J. Rossi et al., "An Alternate Method for Synthesis of Double-Stranded DNA Segments", The J. of Biol. Chem. (1982) 257(16):9226-9229.
T. Kempe et al., Nucleic Acids Research, "Chemical and Enzymatic biotin-labelling of oligodeoxyribonucleotides," vol. 13, No. 1, 1985, pp. 45-57.
R. K. Saiki et al., Science, "Enzymatic amplication of beta-globin genomic sequences and restrictions site analysis for diagnosis of sickle cell anemia," vol. 230, Dec., 1985, pp. 1350-1354.

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