Labeled protein and its producing method, labeling compound...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C530S402000

Reexamination Certificate

active

07041446

ABSTRACT:
A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided. Also, the labeling compound and a method for analyzing a function of a gene are further provided.

REFERENCES:
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patent: 6660473 (2003-12-01), Lohse et al.
patent: 0 962 527 (1999-12-01), None
patent: 98/16636 (1998-04-01), None
“Program and Proceedings of the 20thAnnual Meeting of the Molecular Biology Society of Japan”, Nov. 15, 1997, pp. 121, 546 and 547, together with a summarized translation.
Progress in Biophysics and Molecular Biology, XIIth International Biophysics Congress, abstract No. P-A5-04 (Aug. 1996).
Promega Technical Bulletin, No. 182 (Sep. 1993).
N. Nemoto et al.,FEBS Letters, 414, 405-408 (1997).
R. Vince et al., “Photoaffinity Labeling of the Ribosomal Peptidyl Transferase Site with Synthetic Puromycin Analogues”, Biochemistry, vol. 17, No. 25, pp. 5489-5493, 1978.

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